Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-05T01:06:36.714Z Has data issue: false hasContentIssue false
  • English
  • Français

Proteolytic activity in Caenorhabditis elegans : soluble and insoluble fractions

Published online by Cambridge University Press:  11 April 2024

C. Gimenez-Pardo ,
C. Vazquez-Lopez ,
C. de Armas-Serra  and
F. Rodriguez-Caabeiro
C. Gimenez-Pardo*
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid–Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain
*
*Fax: 91 8854636 E-mail: [email protected]á.es
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Proteolytic activities of soluble and insoluble fractions of the free-living soil nematode Caenorhabditis elegans were measured across a range of substrates, temperatures and pH conditions. Several protease inhibitors were also tested under these conditions. Results of these studies indicate that proteolytic activity is present in cytosolic (CF) and non-cytosolic (NCF) fractions of C. elegans extracts at every condition of pH, temperature and buffer assayed. On the other hand, the use of different protease inhibitors demonstrated the existence of exo and endoproteases types in CF as well as NCF. Moreover our results show that the use of two protease inhibitor mixed types proposed by several authors are not enough to avoid this lytic activity when homogenates of this nematode are employed in biochemical assays.

Type
Review Article
Information
Journal of Helminthology , Volume 73 , Issue 2 , February 1999 , pp. 123 - 127
Copyright
Copyright © Cambridge University Press 1999

References

Armas-Serra, C. de., Giménez-Pardo, C., Jiménez-González, A., Bernadina, W.E. & Rodríguez-Caabeiro, F. (1995) Purification and preliminary characterization of a protease from the excretion–secretion products of Trichinella spiralis muscle larvae. Veterinary Parasitology 59, 157–168.CrossRefGoogle Scholar
Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71–94.CrossRefGoogle ScholarPubMed
Cox, G.N., Pratt, D., Hageman, R. & Boisvenue, R.J. (1990) Molecular cloning and primary sequence of a cysteine protease expressed by Haemonchus contortus adult worms. Molecular and Biochemical Parasitology 41, 25–34.CrossRefGoogle ScholarPubMed
Criado-Fornelio, A., Armas-Serra, C., de. Giménez-Pardo, C., Casado-Escribano, N., Jiménez-González, A. & Rodríguez-Caabeiro, F. (1992) Proteolytic enzymes from Trichinella spiralis larvae. Veterinary Parasitology 45, 133–140.CrossRefGoogle ScholarPubMed
Cross, K. & Barry, D. (1995) A coat for all seasons: the surface of a model nematode. TRP3:. Research and Funding News from Wellcome Trust 3, 19–21.Google Scholar
Epstein, H.F. & Liu, F. (1995) Proteins and protein assemblies. Methods in Cell Biology, 48, 437–450.CrossRefGoogle ScholarPubMed
Kingston, I.B. & Petitt, J. (1990) Structure and expression of Ascaris suum collagen genes: a comparison with Caenorhabditis elegans. Acta Tropica 47(5–6), 283–287.CrossRefGoogle Scholar
Lackey, A., James, E.R., Sakanari, J.A., Resnick, S.D., Brown, M., Bianco, A.E. & Sakanari, J.H. (1989) Extracellular proteases of Onchocerca. Experimental Parasitology 68, 176–185.CrossRefGoogle ScholarPubMed
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Peterson, G.L. (1983) Determination of total protein. Methods in Enzymology 91, 95–119.CrossRefGoogle ScholarPubMed
Richer, J.K., Sakanari, J.A., Frank, G.R. & Grieve, R.B. (1992) Dirofilaria immitis: proteases produced by third and fourth-stage larvae. Experimental Parasitology 75, 213–222.CrossRefGoogle ScholarPubMed
Rohrer, S.P., Evans, D.V. & Bergstrom, A. (1990) A membrane associated glutamate binding protein from Caenorhabditis elegans and Haemonchus contortus. Comparative Biochemistry and Physiology 95, 223–228.Google ScholarPubMed
Salvesen, G. & Nagase, H. (1989) Inhibition of proteolytic activity. pp. 83–105 in Benyon, R.J. & Bond, J.S. (Eds) Proteolytic enzymes: a practical approach. IRL Press.Google Scholar
Sarkis, G.J., Kurpiewski, M.R., Ashcom, J.D., Jen-Jacobson, L. & Jacobson, L.A. (1988) Proteases of the nematode Caenorhabditis elegans. Archives of Biochemistry and Biophysics 261, 80–90.CrossRefGoogle ScholarPubMed
Schaeffer, J.M. & Haines, H.W. (1990) Avermectin binding in Caenorhabditis elegans a two-state for the avermectin binding site. Biochemical Pharmacology 38, 2329–2338.CrossRefGoogle Scholar
Ward, S. (1988) Caenorhabditis elegans: a model for parasite nematodes. in England, P.T. & Sher, A.J. (Eds) The biology of parasitism. A molecular and immunological approach. New York, Alan R. Liss, Inc.Google Scholar