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Proline modulates the effect of bisphosphonate on calcium levels and adenosine triphosphate production in cell lines derived from bovine Echinococcus granulosus protoscoleces

Published online by Cambridge University Press:  07 June 2013

A.G. Fuchs ,
C.I. Echeverría ,
F.G. Pérez Rojo ,
E.A. Prieto González  and
E.J.A. Roldán
A.G. Fuchs*
Affiliation:
Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, (C127OAAH), Buenos Aires, Argentina
C.I. Echeverría
Affiliation:
Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, (C127OAAH), Buenos Aires, Argentina
F.G. Pérez Rojo
Affiliation:
Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, (C127OAAH), Buenos Aires, Argentina
E.A. Prieto González
Affiliation:
Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, (C127OAAH), Buenos Aires, Argentina
E.J.A. Roldán
Affiliation:
Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Universidad Abierta Interamericana (UAI), Avenida Montes de Oca 745, (C127OAAH), Buenos Aires, Argentina
*
*Fax: (54) 11 4301 5240 extension 2 E-mail: [email protected]
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Abstract

Bisphosphonates have been proposed as pharmacological agents against parasite and cancer cell growth. The effect of these compounds on helminthic cell viability and acellular compartment morphology, however, has not yet been studied. The effects of different types of bisphosphonates, namely etidronate (EHDP), pamidronate (APD), alendronate (ABP), ibandronate (IB) and olpadronate (OPD), and their interaction with amiloride, 1,25-dihydroxycholecalciferol (D3) and proline were evaluated on a cell line derived from bovine Echinococcus granulousus protoscoleces (EGPE) that forms cystic colonies in agarose. The EGPE cell line allowed testing the effect of bisphosphonates alone and in association with other compounds that could modulate calcium apposition/deposition, and were useful in measuring the impact of these compounds on cell growth, cystic colony formation and calcium storage. Decreased cell growth and cystic colony formation were found with EHDP, IB and OPD, and increased calcium storage with EHDP only. Calcium storage in EGPE cells appeared to be sensitive to the effect of amiloride, D3 and proline. Proline decreased calcium storage and increased colony formation. Changes in calcium storage may be associated with degenerative changes of the cysts, as shown in the in vitro colony model and linked to an adenosine triphosphate (ATP) decrease. In conclusion, bisphosphonates could be suitable tempering drugs to treat cestode infections.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 4 , December 2014 , pp. 459 - 467
Copyright
Copyright © Cambridge University Press 2013 

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