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Proliferation of pronephric lymphocytes of carp, Cyprinus carpio induced by extracts of Bothriocephalus acheilognathi

Published online by Cambridge University Press:  05 June 2009

P. Nie ,
D. Hoole  and
C. Arme
P. Nie
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, University of Keele, Keele, Staffordshire ST5 5BG, UK
D. Hoole*
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, University of Keele, Keele, Staffordshire ST5 5BG, UK
C. Arme
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, University of Keele, Keele, Staffordshire ST5 5BG, UK
*
†Author for correspondence.
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Abstract

The interaction between Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda)and pronephric lymphocytes of carp, Cyprinus carpio L. was studied by examining proliferation of lymphocytes isolated from both naïve fish and fish injected intraperitoneally with cestode extract. Lymphocytes from naïve hosts were stimulated to proliferate in the presence of the extract depending upon the extract protein concentrations; lower concentrations (0.01–0.05μg/ml) induced the greatest response, and immunosuppression occurred at higher concentrations. Significant differences were noted in fish that received intraperitoneal injections of parasite extracts. Five days post-injection, lymphocyte proliferation was significantly greater in these individuals compared with sham injected or untreated controls. This difference was reduced at 10 days post-injection, although the response was dependent on the concentration of the parasite extract. The possible significance of the observed stimulation/suppression of lymphocyte activity to establishment of the parasite in the wild is discussed.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 2 , June 1996 , pp. 127 - 131
Copyright
Copyright © Cambridge University Press 1996

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