Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-25T23:45:36.087Z Has data issue: false hasContentIssue false
  • English
  • Français

Preliminary Observations on the Golorimetric Assay of the Hatching Factor of the Potato-root Eelworm, Heterodera rostochiensis Wollenweber

Published online by Cambridge University Press:  05 June 2009

C. Ellenby
C. Ellenby
Affiliation:
Department of Zoology, King's College, (University of Durham), Newcastle-upon- Tyne
Get access Rights & Permissions [Opens in a new window]

Extract

Promising results in the colorimetric assay of potato-root eelworm hatching factor have been obtained with picric acid and with 3: 5- dinitrobenzoic acid. Both of the reagents are used for the assay of cardiac glycosides with which the hatching factor may have affinities.

Type
Research Article
Information
Journal of Helminthology , Volume 32 , Issue 4 , December 1958 , pp. 219 - 226
Copyright
Copyright © Cambridge University Press 1958

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Calam, C. T., Raistrick, H. and Todd, A. R., 1949.—“The potato eehvorm hatching factor. 1. The preparation of concentrates and a method of bioassay.” Biochem. J., 45, 513–519. (W.L. 2907).CrossRefGoogle Scholar
Ellenby, C. and Gilbert, A. B., 1957.—“Cardiotonic activity of the potato-root eelworm hatching factor.” Nature, Load., 180, 1105–1106. (W.L. 14900).CrossRefGoogle ScholarPubMed
Ellenby, C., 1958a.—“Root diffusates of Solatium tuberosum and Digitalis pupurea.”ibidem, 181, 920–921.Google Scholar
Ellenby, C., 1958b.—“Solutions of potato-root diffusates of low ion content.” Experientia, 14, 109. (W.L. 8095a).CrossRefGoogle Scholar
Fenwick, D. W., 1952.—“The bioassay of potato-root diffusate.” Ann. appl. Biol., 39. 457–467. (W.L. 1025).CrossRefGoogle Scholar
Katznelson, H., Rouatt, J. W. and Payne, T. B. M., 1955.—“The liberation of amino acids and reducing compounds by plant roots.” Plant and Soil, 7, 35–48.CrossRefGoogle Scholar
Knudsen., A. and Dresbach, M., 1923. “A chemical method of assaying the active principles of Digitalis.” J. Pharmacol., 20, 205–220. (W.L. 11442).Google Scholar
Lyon, T. L., AND Wilson, J. K., 1921.—“Liberation of organic matter by roots of growing plants.” Mem. Cornell Agric. Exp. Sta., 40, 144. (W.L. 13370).Google Scholar
Marrian, D. H., Russell, P. P., Todd, A. R. and Waring, W. S., 1949.—“The potato eelworm hatching factor. 3. Concentration of the factor by chromatography. Observations on the nature of eclepic acid.” Biochem, J., 45, 524–528. (W.L. 2967).CrossRefGoogle Scholar
“MARTINDALE”, 1943.—The extra Pharmacopoeia. London, The Pharmaceutical Press.Google Scholar
Rowson, J. M., 1952.—“Studies in the genus Digitalis. Part 1. The colorimetric estimation of digitoxin and of preparations of Digitalis purpurea.” J. Pharm.Lond., 4, 814–830. (W.L. 11442a).CrossRefGoogle Scholar
Rowson, J. M., 1955.—“Studies in the genus Digitalis. Part 3. The extraction and evaluation of Digitalis purpurea leaf” J. Pharm.Lond., 7, 924–931.Google Scholar
Rowson, J. M. and Dyer, F. J., 1952.—“Studies in the genus Digitalis. Part 2. A comparison of the colorimetric and biological methods for the evaluation of Digitalis purpurea.” J. Pharm.Lond., 4, 831–843.Google Scholar