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Predatory activity of nematophagus fungus Duddingtonia flagrans in infective larvae after gastrointestinal transit: biological control in pasture areas and in vitro

Published online by Cambridge University Press:  14 June 2021

Barbara Haline Buss Baiak Open the ORCID record for Barbara Haline Buss Baiak [Opens in a new window] ,
Jennifer Mayara Gasparina ,
Letícia Ianke ,
Karolini Tenffen de Sousa ,
Matheus Deniz ,
Leticia Macedo Pereira ,
Jackson Victor Araújo ,
Raquel Abdallah da Rocha  and
João Ricardo Dittrich
Barbara Haline Buss Baiak*
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, 80035-050, Paraná, Brazil
Jennifer Mayara Gasparina
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Estadual de Ponta Grossa, Ponta Grossa, 84030-900, Paraná, Brazil
Letícia Ianke
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Estadual de Ponta Grossa, Ponta Grossa, 84030-900, Paraná, Brazil
Karolini Tenffen de Sousa
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, 80035-050, Paraná, Brazil
Matheus Deniz
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, 80035-050, Paraná, Brazil
Leticia Macedo Pereira
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, 80035-050, Paraná, Brazil
Jackson Victor Araújo
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, 36570-000, Minas Gerais, Brazil
Raquel Abdallah da Rocha
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Estadual de Ponta Grossa, Ponta Grossa, 84030-900, Paraná, Brazil
João Ricardo Dittrich
Affiliation:
Programa de Pós-graduação em Zootecnia, Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, 80035-050, Paraná, Brazil
*
Author for correspondence: Barbara Haline Buss Baiak E-mail: [email protected]
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Abstract

Biological control is a strategy to decrease parasitic populations, and the action takes place through natural antagonists in the environment. We studied the predatory activity of the fungus Duddingtonia flagrans in infective larvae (L3) of gastrointestinal nematodes after gastrointestinal transit. Ten heifers were divided into two groups: treated (animals received pellets containing fungus) and control (animals received pellets without fungus). Twelve hours after administration, faeces samples were collected for in vitro efficacy tests. The animals then remained for 7 h in the experimental pasture area. At the end of this period, 20 faecal pads (ten treated and ten control) were selected at random. Pasture, faecal pad and soil collections occurred with an interval of 7 days, totalling four assessments. In vitro activity demonstrated that fungi effectively preyed on L3, achieving a reduction percentage of 88%. In the faecal pad of the pasture area, there was a difference (P < 0.05) between collections 3 and 4 for both groups; in the treated group a reduction of 65% was obtained, while in the control group there was an increase of 217% in the number of L3. The recovery of L3 in the soil and in the pasture was similar in both groups. There was no influence (P = 0.87) of the passage time on the fungus predatory activity. Duddingtonia flagrans demonstrated the ability to survive gastrointestinal transit in the animals, reducing the number of L3 in the faeces, indicating that this biological control has great potential in the control of worm infections.

Keywords

Biological controlheifersworms
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e31
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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