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Polarographic determination of mitochondrial respiration in Gastrothylax crumenifer

Published online by Cambridge University Press:  05 June 2009

Jawed Siddiqui  and
Ather H Siddiqi
Jawed Siddiqui
Affiliation:
Section of Parasitology, Deparment of Zoology, Aligarh Muslim University, Aligarh-202 001, India
Ather H Siddiqi
Affiliation:
Section of Parasitology, Deparment of Zoology, Aligarh Muslim University, Aligarh-202 001, India
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Abstract

The oxidative metabolism of Gastrothylax crumenifer mitochondrial fractions was assayed polarographically at 30°C in the presence of various substrates. Succinate was most readily oxidized, malate and pyruvate were oxidized at subsantially lower retes. The spcific inhibitors such as malonate, KCN, NaN3, oxaloacetate, salicylhydroxamic acid and oligomycin effectively affected the mitochondrial oxygen consumption. The results indicate the presence of oxidative phosphorylation and cytochrome systems. Mitochondrial ATPase was found to be active.

Keywords

Gastrothylax crumeniferrespirationoxidative phosphorylation
Type
Research Note
Information
Journal of Helminthology , Volume 61 , Issue 2 , June 1987 , pp. 143 - 145
Copyright
Copyright © Cambridge University Press 1987

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References

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