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On the identification of some species of Trichostrongylus

Published online by Cambridge University Press:  18 November 2009

Phyllis A. Clapham
Phyllis A. Clapham
Affiliation:
From the Institute of Agricultural Parasitology, St. Albans.
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Extract

A method is described by means of which trichostrongylid spicules are separated from the surrounding soft tissues and their examination made easier. NaOH and KOH are the most satisfactory substances for this purpose, though both substances eventually act on the chitin of the spicules themselves, causing distortion. The reaction takes place at a temperature of 37°C. with reasonable speed. Spicules of Ostertagia can also be freed satisfactorily by this method but those of Nematodirus are quickly affected by the alkali, with consequent distortion.

Type
Research Papers
Information
Journal of Helminthology , Volume 22 , Issue 1 , November 1947 , pp. 37 - 46
Copyright
Copyright © Cambridge University Press 1947

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