Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-24T23:22:14.183Z Has data issue: false hasContentIssue false
  • English
  • Français

The nature of antigens common to both the metacestodes of Taenia crassiceps and its laboratory host

Published online by Cambridge University Press:  18 November 2009

J. Chernin
J. Chernin
Affiliation:
Department of Biological Sciences, North East Surrey College of Technology, Reigate Road, Ewell, Surrey, England
Get access Rights & Permissions [Opens in a new window]

Abstract

Metacestodes of Taenia crassiceps were cultured in laboratory rats and mice, washed in glycine/hydrochloric acid (Gly/Hcl) buffer pH 2·4 and phosphate buffered saline (PBS) pH 7·2 and a soluble extract of the metacestodes (SEM) was prepared from larvae washed in both buffers. The SEM and the original washings were examined for host IgG and a common host antigen (CHA). CHA was present in both the SEM and Washings but IgG only in the washings and, in metacestodes from mice only in the Gly/HCl washings). Metacestodes washed in Gly/HCl were not viable.

Metacestodes were also maintained in vitro in a peptide nutrient agar and 0·9% saline at 37°C and a SEM was prepared from these; metacestodes from mice had neither host IgG nor CHA but those from rats had CHA but not IgG. SEM prepared from ‘normal’ metacestodes showed that the CHA consisted of at least two entities, only one having a common identity with the IgG. These results suggest that molecules of both host IgG and a common host antigen are present on the tegument of these parasites.

Type
Research Papers
Information
Journal of Helminthology , Volume 56 , Issue 4 , December 1982 , pp. 339 - 344
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Capron, A., Biguet, J., Rose, F. & Vernes, A. (1965) Les antigènes de Schistosoma mansoni. II. Etude immunoelectrophorétique comparée de divers stades larvaires et des adultes des deux sexes. Aspects immunologiques des relations hôte-parasite de la cercaire et de l'adulte de Schistosoma mansoni. Annales de l'Institut Pasteur, 109, 798–810.Google Scholar
Chernin, J. (1977) Common host antigens in laboratory rats infected with the metacestodes of Taenia crassiceps. Journal of Helminthology, 51, 215–220.Google Scholar
Chernin, J. (1981) The growth and antigenicity of the metacestodes of Taenia crassiceps in several different strains of rats. Journal of Helminthology, 55, 209–222.Google Scholar
Damian, R. T. (1967) Common antigens between adult Schistosoma mansoni and the laboratory mouse. Journal of Parasitology, 53, 60–64.Google Scholar
Hustead, S. T. & Williams, J. F. (1977) Permeability studies on taeniid metacestodes: 1. Uptake of proteins by larval stages of Taenia taeniaformis, T. crassiceps and Echinococcus granulosus. Journal of Parasitology, 63, 314–321.CrossRefGoogle Scholar
Macinnes, A. J. & Voge, M. (1970) Experiments in Parasitology. W. H. Freeman & Company: Chicago.Google Scholar
McGreevy, P. B., Ismail, M. M., Phillips, T. M. & Denham, D. A. (1975) Studies with Brugia pahangi 10. Journal of Helminthology, 49, 107–113.Google Scholar
Smithers, S. R., Terry, R. J. & Hockley, O. S. (1969) Host antigens in schistosomiasis. Proceedings of the Royal Society B, 171, 483–494.Google Scholar
Sogandares-Bernal, F. & Voge, M. (1978) Immunoglobins on the surfaces of tetrathyridia of Mescestoides corti Hoeppli 1925 (Cestoda), from laboratory infections of ICR mice. Journal of Parasitology, 64, 620–624.CrossRefGoogle Scholar