Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-20T00:45:22.411Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphometric and molecular characterizations of schistosome populations in Estuaire province Gabon

Published online by Cambridge University Press:  22 July 2009

R. Mintsa Nguema ,
K. Mengue Ngou Milama ,
M. Kombila ,
D. Richard-Lenoble ,
P. Tisseyre ,
M. Ibikounlé ,
H. Moné  and
G. Mouahid
R. Mintsa Nguema
Affiliation:
Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5244 CNRS-EPHE-UPVD, Université de Perpignan, Perpignan, France
K. Mengue Ngou Milama
Affiliation:
Service de Parasitologie-Mycologie-Médecine Tropicale, Faculté de Médecine de Tours, CHRU Bretonneau, Tours, France
M. Kombila
Affiliation:
Département de Parasitologie-Mycologie, Faculté de médecine, Université des Sciences de la Santé, Libreville, Gabon
D. Richard-Lenoble
Affiliation:
Service de Parasitologie-Mycologie-Médecine Tropicale, Faculté de Médecine de Tours, CHRU Bretonneau, Tours, France
P. Tisseyre
Affiliation:
Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5244 CNRS-EPHE-UPVD, Université de Perpignan, Perpignan, France
M. Ibikounlé
Affiliation:
Département de Zoologie et Génétique, Laboratoire de Parasitologie et d'Ecologie parasitaire, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, Cotonou, Bénin
H. Moné
Affiliation:
Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5244 CNRS-EPHE-UPVD, Université de Perpignan, Perpignan, France
G. Mouahid*
Affiliation:
Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5244 CNRS-EPHE-UPVD, Université de Perpignan, Perpignan, France
*
*Fax: +33 468 662 281 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of this study was to test the hypothesis of the presence of hybrids between Schistosoma guineensis and S. haematobium in the Estuaire province (Western Gabon). Egg morphometry and single-stranded conformational polymorphism (SSCP) analysis on adult worms were used in order to characterize the schistosome populations of two sites. The morphology of the eggs showed three morphotypes: S. haematobium, S. guineensis and intermediate morphotypes, but the eggs of the morphotype S. guineensis were smaller compared to the values found in the literature. Furthermore, the SSCP analysis of the adult schistosomes showed that all the patterns corresponded to that of S. haematobium and gave evidence that hybrids were absent from our samples.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 1 , March 2010 , pp. 81 - 85
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brown, D.S., Sarfati, C., Southgate, V.R., Ross, G.C. & Knowles, R.J. (1984) Observations on Schistosoma intercalatum in South-East Gabon. Zeischrift für Parasitenkunde 70, 243–253.CrossRefGoogle ScholarPubMed
Burchard, G.D. & Kern, P. (1985) Probable hybridization between S. intercalatum and S. haematobium in Western Gabon. Tropical and Geographical Medicine 37, 119–123.Google Scholar
Gilles, J.C. (1971) Les bilharzioses au Gabon. Bulletin de la Société de Pathologie Exotique 64, 879–886.Google ScholarPubMed
Kane, R.A., Bartley, J., Stothard, J.R., Vercruysse, J., Rollinson, D. & Southgate, V.R. (2002) Application of single strand conformational polymorphism (SSCP) analysis with fluorescent primers for differentiation of Schistosoma haematobium group species. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 235–241.CrossRefGoogle ScholarPubMed
Loker, E.S. (1983) A comparative study of the life-histories of mammalian schistosomes. Parasitology 87, 343–369.CrossRefGoogle ScholarPubMed
Mutani, A., Christensen, N.Ø. & Frandsen, F. (1985) A study of the biological characteristics of a hybrid line between male Schistosoma haematobium (Dar es Salaam, Tanzania) and female S. intercalatum (Edea, Cameroun). Acta Tropica 42, 319–331.Google Scholar
Pagès, J.R., Jourdane, J., Southgate, V.R. & Tchuem Tchuenté, L.A. (2003) Reconnaissance de deux espèces jumelles au sein du taxon Schistosoma intercalatum (Fisher, 1934), agent de la schistosomose humaine rectale. Description de S. guineensis n. sp. pp. 139–146 in Combes, C. & Jourdane, J. (Eds) Taxonomie, écologie et évolution des métazoaires parasites (2). Perpignan, Presses Universitaires.Google Scholar
Richard-Lenoble, D., Kombila, M., Duong, T.H. & Gendrel, D. (1993) Bilharziose à Schistosoma intercalatum, bilharziose récente et oubliée. Revue du Praticien 43, 432–439.Google Scholar
Southgate, V.R., Van Wijk, H.B. & Wright, C.A. (1976) Schistosomiasis at Loum Cameroon: S. haematobium, S. intercalatum and their natural hybrid. Zeischrift für Parasitenkunde 49, 145–159.CrossRefGoogle ScholarPubMed
Webster, B.L., Tchuem Tchuenté, L.A., Jourdane, J. & Southgate, V.R. (2005) The interaction of Schistosoma haematobium and S. guineensis in Cameroon. Journal of Helminthology 79, 193–197.CrossRefGoogle ScholarPubMed
Webster, B.L., Tchuem Tchuenté, L.A. & Southgate, V.R. (2007) A single-strand conformation polymorphism (SSCP) approach for investigating genetic interactions of Schistosoma haematobium and Schistosoma guineensis in Loum, Cameroon. Parasitology Research 100, 739–745.CrossRefGoogle ScholarPubMed
Wright, C.A., Southgate, V.R. & Knowles, R.J. (1972) What is Schistosoma intercalatum Fisher, 1934? Transactions of the Royal Society of Tropical Medicine and Hygiene 66, 28–64.CrossRefGoogle ScholarPubMed