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Morphological differences between Venezuelan and African microfilariae of Onchocerca volvulus

Published online by Cambridge University Press:  05 June 2009

C. Botto ,
A. Escalante ,
M. Arango  and
L. Yarzabal
C. Botto
Affiliation:
Tropical Medicine Institute/Central University of Venezuela/UCV/Apartado 2109/Caracas
A. Escalante
Affiliation:
Department of Environmental Studies/Simón Bolívar University/Sertanejas
M. Arango
Affiliation:
Amazonian Center for Research and Control of Tropical Diseases/CAICET/Puerto Ayacucho/Venezuela
L. Yarzabal
Affiliation:
Amazonian Center for Research and Control of Tropical Diseases/CAICET/Puerto Ayacucho/Venezuela
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Abstract

Comparative morphological and biometric characteristics of microfilariae of Onchocerca gutturosa and O. volvulus from different geographical areas (Upper Orinoco, Venezuela; Togo; Liberia) were assessed. “Stepwise” discriminant analysis and Mahalanobis estimators were applied to measure distance between populations. The results indicate a strong similarity between the two strains from the Upper Orinoco (Venezuela) and the Togo strain, as well as a clear separation between these strains and that of O. gutturosa. The Liberian strain was easily distinguishable from microfilariae from Togo and Venezuela. Discriminant analysis showed the Liberian deme to be as different from the Venezuelan and Togo demes as these demes were from microfilariae of the reference species, O. gutturosa. Although it is necessary to confirm these data using formalin-fixed specimens obtained from the skin, the present findings suggest the existence of geographically-different strains of O. volvulus in America and Africa.

Keywords

Onchocerca volvulusmicrofilariaediscriminant analysisUpper OrinocoTogoLiberia
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 4 , December 1988 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 1988

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References

Bain, O. (1975) Rédescription de cinq espèces d'onchocerques. Annales de Parasitologie Humaine et Comparée, 50, 763–788.CrossRefGoogle Scholar
Bain, O. (1981) Le genre Onchocerca: Hypothèses sur son évolution et clé dichotomique des espèces. Annales de Parasitologie Humaine et Comparée, 56, 503–526.Google Scholar
Black, G. A. (1984) Morphometrics of Cystidicola stigmatura (Nematoda) in relation to its glacial story. Journal of Parasitology, 70, 967–974.Google Scholar
Blacklock, D. B. (1926) The development of Onchocerca volvulus in Simulium damnosum. Annals of Tropical Medicine and Parasitology, 20, 1–48.Google Scholar
Botto, C., Arango, M. & Yarzabal, L. (1984) Onchocerciasis in Venezuela: Prevalence of microfilaraemia in amerindians and morphological characteristics of the microfilariae in the Upper Orinoco Focus. Tropenmedizin und Parasitologie, 35, 167–173.Google Scholar
Botto, C., Sampson, L., Arango, M., Ayesta, C., Belmonte, P. & Yarzabal, L. (1985a) Estudio morfológico comparative de microfilarias de Onchocerca volvulus de Liberia, Togo y Venezuela (Comunicación preliminar). In: La Oncocercosis en América (Edited by Caicet) Publicación Científica No 3, 125–138.Google Scholar
Botto, C., Arango, M., Sampson, L., Belmonte, P., Ayesta, C. & Yarzabal, L. (1985b) Influencia de los métodos de fijación sobre el diagnóstico de la microfilaremia en la oncocercosis. In: La Oncocercosis en América (edited by Caicet) Publicación Científica No 30, 113–124.Google Scholar
Campbell, N. A. (1976) A multivariate approach to variation in microfilariae: examination of the species Wuchereria lewisi and demes of the speciesW. bancrofti. Australian Journal of Zoology, 24, 105–114.CrossRefGoogle Scholar
Colless, D. H. (1971) An application of numerical methods to the systematics of microfilariae. Annals of Tropical Medicine and Parasitology, 65, 245–251.CrossRefGoogle Scholar
Cianchi, R., Karam, M., Henry, M. C., Villani, F., Kumlien, S. & Bullini, L. (1985) Preliminary data on the genetic differentiation of Onchocerca volvulus in Africa (Nematoda: Filarioidea). Acta Tropica 42, 341–351.Google Scholar
Cuadras, C. M. (1981) Métodos de Análisis Multivariante. Edited by Eunibar, Barcelona.Google Scholar
De Leon, J. R. & Duke, B. O. L. (1966) Experimental studies on the transmission of Guatemalan and West African strains of Onchocerca volvulus by Simulium ochraceum, S. metallicum and S. callidum. Transactions of the Royal Society of Tropical Medicine and Hygiene, 60, 735–752.Google Scholar
Duke, B. O. L., Lewis, D. J. & Moore, P. J. (1966) Onchocerca–Simulium complexes I. Transmission of forest and Sudan-savanna strains of Onchocerca volvulus, from Cameroon, by Simulium damnosum from various west African bioclimatic zones. Annals of Tropical Medicine and Parasitology, 60, 318–336.Google Scholar
Duke, B. O. L. (1970) Onchocerca-Simulium complexes. VI. Experimental studies on the transmission of Venezuelan and West African strains of Onchocerca volvulus by Simulium metallicum and S. exiguum in Venezuela. Annals of Tropical Medicine and Parasitology, 64, 421–431.Google Scholar
Eichler, D. A. (1968) Size variation of the microfilariae of Onchocerca gutturosa Neumann. Transactions of the Royal Society of Tropical Medicine and Hygiene, 62, 467–468.Google Scholar
Erttmann, K. D., Unnasch, T. R., Greene, B. M., Albiez, E. J., Boateng, J., Denke, A. M., Ferraroni, J. J., Karam, M., Schulz-Key, H. & Williams, P. N. (1987) A DNA sequence specific for forest form Onchocerca volvulus. Nature, 327, 415–417.CrossRefGoogle ScholarPubMed
Escalante, A. (1985) Estudio comparativo de demos neotropicales de Onchocerca volvulus Leuckart, 1893. Tesis de Licenciatura en Biología. Edited by Universidad Simón Bolivar, Caracas.Google Scholar
Flockhart, H. A., Cibulskis, R. E., Karam, M. & Albiez, E. (1986) Onchocerca volvulus: enzyme polymorphism in relation to the differentiation of forest and savannah strains of this parasite. Transactions of the Royal Society of Tropical Medicine and Hygiene, 80, 285–292.CrossRefGoogle Scholar
Franz, M. (1980) Electron microscope study of the cuticle of male and female Onchocerca volvulus from various geographic areas. Tropenmedizin und Parasitologie, 31, 149–164.Google ScholarPubMed
Gibson, C. L. (1952) Comparative morphology of the skin inhabiting microfilariae of man, cattle and equines in Guatemala. The American Journal of Tropical Medicine and Hygiene, 1, 250–261.Google Scholar
Gratama, S. (1970) Microfilaria bancrofti and Microfilaria volvulus in hydrocele fluid and tissue sections. Morphology and differential diagnosis. Tropical and Geographical Medicine, 22, 459–470.Google Scholar
Hirai, H., Tada, I., Takahashi, H., Nwoke, B. E. & Ufomadu, G. O. (1987) Chromosomes of Onchocerca volvulus (Spirurida: Onchocercidae): a comparative study between Nigeria and Guatemala. Journal of Helminthology, 61, 43–46.CrossRefGoogle ScholarPubMed
Laurence, B. R. & Simpson, M. G. (1968) Cephalic and pharyngeal structures in microfilariae revealed by staining. Journal of Helminthology, 42, 309–330.Google Scholar
Lobos, E. & Weiss, N. (1985) Immunochemical comparison between worm extracts of Onchocerca volvulus from savanna and rain forest. Parasite Immunology, 7, 333–347.Google Scholar
Martinez-Baez, M. (1976) Nueva descripción de la Microfilaria de Onchocerca volvulus (Leuckart 1894) Railliet y Henry 1910, procedente de Chiapas, México. Revista de Investigación en Salud Pública, 36, 139–150.Google Scholar
Omar, M. S. (1978) Histochemical enzyme staining patterns of Onchocerca volvulus and their occurrence in different onchocerciasis areas. Tropenmedizin und Parasitologie, 29, 462–472.Google ScholarPubMed
Schacher, J. F. & Geddawi, M. (1969) An analysis of speciation and evolution in Wuchereria bancrofti by the study of nuclear constancy (eutely) in microfilariae. Annals of Tropical Medicine and Parasitology, 63, 67–82.Google Scholar
Schiller, E., Turner, V., Marroquin, H. & D'antonio, R. (1979) The cryopreservation and in vitro cultivation of larval Onchocerca volvulus. The American Journal of Tropical Medicine and Hygiene, 28, 997–1009.Google Scholar
Takaoka, H., Tada, I., Hashiguchi, Y., Baba, M., Korenaga, M., Onofre, J., Convit, J. & Yarzabal, L. (1986) A cross compatibility study of Guatemalan and North Venezuelan Onchocerca volvulus to Simulium metallicum from two countries. Japanese Journal of Parasitology, 35, 35–41.Google Scholar
WHO (1987) Third Report. WHO Expert Committee on Onchocerciasis. Technical report Series, 752, 1–167.Google Scholar
Woo, P. T. & Black, G. A. (1984) Trypanosoma danilewsky: host specificity and host effect on morphometrics. Journal of Parasitology, 70, 788–793.CrossRefGoogle Scholar
Yarzabal, L., Petralanda, I., Arango, M., Lobo, L. & Botto, C. (1983a) Acid phosphatase patterns in microfilariae of Onchocerca volvulus s.l from the Upper Orinoco Basin. Tropenmedizin und Parasitologie, 34, 109–113.Google ScholarPubMed