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Morphological comparison of in ovo derived and naturally occurring flukes using Microphallus similis, M. pygmaeus and Maritrema arenaria

Published online by Cambridge University Press:  05 June 2009

L.C. Strain  and
S.W.B. Irwin
L.C. Strain
Affiliation:
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, Co. Antrim, BT37 0QB, Northern Ireland
S.W.B. Irwin*
Affiliation:
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, Co. Antrim, BT37 0QB, Northern Ireland
*
*Author for correspondence.
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Abstract

This investigation deals with the evaluation of the in ovo technique for the cultivation of trematodes. The technique has previously been used for the maturation of digenetic flukes and the establishment of life cycles in the laboratory but it was not known if the adult flukes produced were morphologically comparable to those found in their natural hosts. Three species of microphallids, Microphallus similis, M. pygmaeus and Maritrema arenaria were used. Metacercariae of each species were inoculated into fertile hen's eggs which were incubated at 41°C for 120 hours. The adult flukes recovered were stained and mounted and morphonietric measurements obtained were compared to definitive descriptions of the three microphallid species. The results showed that the in ovo technique provided adult flukes which were indistinguishable from those occurring in natural hosts.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 2 , June 1995 , pp. 155 - 158
Copyright
Copyright © Cambridge University Press 1995

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