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Morphological and molecular characterization of Trophurus wuhuensis n. sp. (Nematoda: Telotylenchinae) from soil associated with Cinnamomum camphora in China

Published online by Cambridge University Press:  04 October 2017

Y. Li
Affiliation:
Department of Plant Pathology, Henan Agricultural University, Zhengzhou 450002, Henan, China
J. Du
Affiliation:
Department of Plant Pathology, Henan Agricultural University, Zhengzhou 450002, Henan, China
Z.Y. Wang
Affiliation:
Department of Plant Pathology, Henan Agricultural University, Zhengzhou 450002, Henan, China
K. Wang*
Affiliation:
Department of Plant Pathology, Henan Agricultural University, Zhengzhou 450002, Henan, China
H.L. Li*
Affiliation:
Department of Plant Pathology, Henan Agricultural University, Zhengzhou 450002, Henan, China Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450002, Henan, China National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, Henan, China
*
Author for correspondence: K. Wang and H.L. Li, E-mails: [email protected]; [email protected]
Author for correspondence: K. Wang and H.L. Li, E-mails: [email protected]; [email protected]

Abstract

A new plant nematode species, Trophurus wuhuensis n. sp., was collected from the soil associated with Cinnamomum camphora in Wuhu, Anhui Province, China. The new species is characterized by having a female with a slender body 660.5–801.5 μm in length, stylet 12–14 μm long, knobs directed laterad, lateral field marked by short and scattered grooves, post-vulval uterine sac shorter than vulval body diameter, post-rectal intestinal sac absent, tail cylindroid, terminus with deep wrinkles; and male with a pointed tail terminus and spicules 16–18 μm long. The internal transcribed spacer sequences of ribosomal DNA (ITS rDNA) and partial 18S ribosomal DNA (18S rDNA) from T. wuhuensis n. sp. were amplified and sequenced. A phylogenetic analysis based on sequences of 18S rDNA fragments is given in this study.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2017 

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Footnotes

Y. Li and J. Du contributed equally to this work.

References

De Waele, D and Bolton, C (1988) Trophurus pakendorfi n. sp. from sunflower in South Africa (Nemata: Telotylenchinae). Phytophylactica 20, 153155.Google Scholar
Ganguly, S and Khan, E (1983) Trophurus impar sp. n. and Scutellonema eclipsi sp. n. (Nematoda: Tylenchida). Indian Journal of Nematology 13, 230234.Google Scholar
Geraert, E (2011) The Dolichodoridae of the world. Identification of the family Dolichodoridae (Nematoda: Tylenchida). 520 pp. Gent, Belgium, Academia Press.Google Scholar
Handoo, ZA, Palomares-Rius, JE, Cantalapiedra-Navarrete, C, Liébanas, G, Subbotin, SA and Castillo, P (2014) Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny. Zoological Journal of the Linnean Society 172, 231264.Google Scholar
Holterman, M, van der Wurff, A, van den Elsen, S, van Megen, H, Bongers, T, Holovachov, O, Bakker, J and Helder, J (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 17921800.Google Scholar
Hooper, DJ, Hallmann, J and Subbotin, SA (2005) Methods for extraction, processing and detection of plant and soil nematodes. pp. 5386 in Luc, M, Sikora, RA and Bridge, J (Eds) Plant parasitic nematodes in subtropical and tropical agriculture. 2nd edn. UK, CABI Publishing.Google Scholar
Huelsenbeck, JP and Ronquist, F (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754755.Google Scholar
Kleynhans, KPN and Cadet, P (1994) Trophurus deboeri n. sp. from sugarcane soil in Barbados and key to the species of the genus Trophurus Loof, 1956 (Nemata: Belonolaimidae). Fundamental and Applied Nematology 17, 225230.Google Scholar
Li, J and Zhao, H (2012) The first report on Trophurus minnesotensis in China. Journal of Qingdao Agricultural University (Natural Science) 29, 1517.Google Scholar
Nylander, JAA (2004) MrModeltest 2.3. Program distributed by the author, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden.Google Scholar
Seinhorst, JM (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 6769.Google Scholar
Sen, D, Chatterjee, A and Manna, B (2012) A new and a known species of Telotylenchinae (Tylenchida: Belonolaimidae) from west Bengal, India. Records of the Zoological Survey of India 112, 2734.Google Scholar
Sher, SA and Bell, AH (1975) Scanning electron micrographs of the anterior region of some species of Tylenchoidea (Tylenchida: Nematoda). Journal of Nematology 7, 6983.Google Scholar
Siddiqi, MR (2000) Tylenchida: Parasites of plants and insects. 2nd edn. 833 pp. Wallingford, UK, CABI Publishing.Google Scholar
Tamura, K, Peterson, D, Peterson, N, Stecher, G, Nei, M and Kumar, S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 27312739.Google Scholar
Vrain, TC, Wakarchuk, DA, Levesque, AC and Hamilton, RI (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology 15, 563573.Google Scholar
Wang, JL, Zhang, JC and Gu, JF (2011) Method of extracting DNA from a single nematode. Plant Quarantine 25, 3235.Google Scholar
Wang, K, Xie, H, Li, Y, Xu, CL, Yu, L and Wang, DW (2013) Paratylenchus shenzhenensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Anthurium andraeanum in China. Zootaxa 3750, 167175.Google Scholar
Wang, K, Xie, H, Li, Y, Wu, WJ and Xu, CL (2016) Morphology and molecular analysis of Paratylenchus nanjingensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Pinus massoniana in China. Journal of Helminthology 90, 166173.Google Scholar