Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-28T17:19:55.078Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae)

Published online by Cambridge University Press:  28 August 2009

K.R. dos Santos ,
B.C. Carlos ,
K.S. Paduan ,
S.M. Kadri ,
T.H. Barrella ,
M.R.V. Amarante ,
P.E.M. Ribolla  and
R.J. da Silva
K.R. dos Santos
Affiliation:
Departamento de Medicina Veterinária, Universidade Federal do Piauí, Campus Cinobelina Elvas, Bom Jesus, Piauí, Brazil
B.C. Carlos
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
K.S. Paduan
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
S.M. Kadri
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
T.H. Barrella
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
M.R.V. Amarante
Affiliation:
Departamento de Parasitologia, Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
P.E.M. Ribolla
Affiliation:
Departamento de Parasitologia, Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
R.J. da Silva*
Affiliation:
Departamento de Parasitologia, Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
*
*Fax: 55 14 38153744 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasitic females. Samples were obtained from the small intestine of Oxyrhopus guibei (Serpentes, Colubridae) collected in the municipality of Lençóis Paulista, State of São Paulo, Brazil. DNA amplification by polymerase chain reaction (PCR) resulted in a 350 bp band for samples containing S. ophidiae and Strongyloides venezuelensis DNA. Strongyloides ophidiae nucleotide sequence analysis showed 98% similarity with Strongyloides procyonis and 97% with Strongyloides cebus, Strongyloides stercoralis, Strongyloides fuelleborni and Strongyloides sp. from snakes.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 2 , June 2010 , pp. 136 - 142
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Dorris, M. & Blaxter, M. (2000) The small subunit ribosomal RNA sequence of Strongyloides stercoralis. International Journal for Parasitology 30, 939–941.CrossRefGoogle ScholarPubMed
Dorris, M., Viney, E.M. & Blaxter, L.M. (2002) Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. International Journal for Parasitology 32, 1507–1517.CrossRefGoogle ScholarPubMed
Eberhardt, A.G., Mayer, W.E., Bonfoh, B. & Streit, A. (2008) The Strongyloides (Nematoda) of sheep and the predominant Strongyloides of cattle form at least two different, genetically isolated populations. Veterinary Parasitology 157, 89–99.CrossRefGoogle ScholarPubMed
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783.Google ScholarPubMed
Grove, D.I. (1989) Strongyloidiasis: a major roundworm infection in man. 336 pp. London, Taylor & Francis.Google Scholar
Hasegawa, H., Hayashida, S., Ikeda, Y. & Sato, H. (2009) Hyper-variable regions in 18S rDNA of Strongyloides spp. as markers for species-specific diagnosis. Parasitology Research 104, 869–874.CrossRefGoogle ScholarPubMed
Hoffmann, R.P. (1987) Diagnóstico de parasitismo veterinário. pp. 33–39. Porto Alegre, Sulina.Google Scholar
Kumar, S., Tamura, K., Jakobsen, I.B. & Nei, M. (2001) MEGA 2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17, 1244–1245.CrossRefGoogle ScholarPubMed
Little, M.D. (1966) Seven new species of Strongyloides (Nematoda) from Lousiana. Journal of Parasitology 52, 85–97.CrossRefGoogle Scholar
Pereira, C. (1929) Strongyloides ophidiae. Boletim Biológico de São Paulo 15, 16–17.Google Scholar
Rodrigues, O.H. (1968) Sobre nova espécie do gênero Strongyloides Grassi, 1879 (Nematoda, Rhabdiasoidea). Atas Sociedade de Biologia 12, 31–32.Google Scholar
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.Google Scholar
Sato, H., Sauzuki, K., Osanai, A., Kamyia, H. & Furuoka, H. (2006) Identification and characterization of the threadworm, Strongyloides procyonis, from feral raccoons (Procyon lotor) in Japan. Journal of Parasitology 92, 63–68.CrossRefGoogle ScholarPubMed
Singh, S.N. (1954) Studies on the morphology and life-history of Strongyloides mirzai n. sp. from snakes in India. Journal of Helminthology 28, 25–34.CrossRefGoogle Scholar
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.C. (1997) CLUSTRAL X: windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Vieira, F.M., Lima, S.S. & Bessa, E.C.A. (2006) Morfologia e Biometria de Strongyloides sp. Grassi, 1879 (Rhabditoidea: Strongyloididae) parasito gastrointestinal de Hydrochaeris hydrochaeris (Linneus, 1766) (Rodentia: Hydrochaeridae) no município de Juiz de Fora, Minas gerais. Revista Brasileira de Parasitologia Veterinária 15, 7–12.Google Scholar
Viney, M.E. & Lok, J.B. (2007) Strongyloides spp. In The C. elegans Research Community (Ed.) WormBook, available athttp://www.wormbook.org (accessed 29 July 2009).CrossRefGoogle Scholar