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Morphological and molecular characterization of larval trematodes infecting the assassin snail genus Anentome in Thailand

Published online by Cambridge University Press:  27 July 2022

N. Chomchoei ,
T. Backeljau ,
B. Segers ,
C. Wongsawad ,
P. Butboonchoo  and
N. Nantarat Open the ORCID record for N. Nantarat [Opens in a new window]
N. Chomchoei
Affiliation:
Ph.D.'s Degree Program in Biodiversity and Ethnobiology (International Program), Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
T. Backeljau
Affiliation:
Royal Belgian Institute of Natural Sciences (BopCo - Barcoding facility for organisms and tissues of policy concern), Vautierstraat 29, B-1000 Brussels, Belgium Evolutionary Ecology Group, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
B. Segers
Affiliation:
Royal Belgian Institute of Natural Sciences (BopCo - Barcoding facility for organisms and tissues of policy concern), Vautierstraat 29, B-1000 Brussels, Belgium
C. Wongsawad
Affiliation:
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Research Center in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
P. Butboonchoo
Affiliation:
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Research Center in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
N. Nantarat*
Affiliation:
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Research Center in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
*
Author for correspondence: N. Nantarat, E-mail: [email protected]
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Abstract

The assassin snail genus Anentome is widespread in Southeast Asia, and is distributed all over the world via the aquarium trade. One species of genus Anentome, Anentome helena, is known to act as intermediate host of parasitic trematodes. This study investigates the taxonomic diversity of larval trematodes infecting A. helena and Anentome wykoffi in Thailand. Larval trematodes were identified by combining morphological and DNA sequence data (cytochrome c oxidase I and internal transcribed spacer 2). Species delimitation methods were used to explore larval trematode species boundaries. A total of 1107 specimens of Anentome sp. were collected from 25 localities in Thailand. Sixty-two specimens of A. helena (n = 33) and A. wykoffi (n = 29) were infected by zoogonid cercariae, heterophyid metacercariae and echinostome metacercariae, with an overall prevalence of 5.6% (62/1107) and population-level prevalences in the range of 0.0–22.3%. DNA sequence data confirmed that the larval trematodes belong to the families Echinostomatidae, Heterophyidae and Zoogonidae. As such, this is the first report of zoogonid cercariae and heterophyid metacercariae in A. helena, and echinostome metacercariae in A. wykoffi. Moreover, this study provides evidence of tentative species-level differentiation between Thai Echinostoma sp. and Cambodian Echinostoma mekongi, as well as within Echinostoma caproni, Echinostoma trivolvis and Echinostoma revolutum.

Keywords

Nassariidaecercariaemetacercariaephylogenetic treespecies delimitation
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e52
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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