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Morphological and molecular analyses of larval trematodes in the intertidal bivalve Perumytilus purpuratus from central Chile

Published online by Cambridge University Press:  19 September 2012

G. Muñoz ,
Z. López  and
L. Cárdenas
G. Muñoz*
Affiliation:
Facultad de Ciencias del Mar y de Recursos Naturales, Universidad de Valparaíso, PO Box 5080, Viña del Mar, Chile
Z. López
Affiliation:
Facultad de Recursos del Mar, Universidad de Antofagasta, PO Box 170, Antofagasta, Chile
L. Cárdenas
Affiliation:
Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile, PO Box 567, Isla Teja, Valdivia, Chile
*
* E-mail: [email protected]
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Abstract

The bivalve Perumytilus purpuratus is a common species that is widely distributed throughout rocky intertidal zones in Chile. This bivalve is the first intermediate host for three trematode species: one bucephalid (an undetermined species) and two fellodistomids (Proctoeces lintoni and one undetermined species). A few studies based on morphological comparisons, experimental infection and molecular analyses have been performed to ascertain the taxon (at least at the family level) to which these trematodes belong; yet, there remains no clarification about the specific identity of these trematodes. Therefore, in this study, we compared the V4 region nucleotide sequences of the 18S rRNA of these three sporocyst species, classified as morphotypes, found in P. purpuratus and nine adult trematode species from intertidal fishes that are likely definitive hosts for these parasites. The sequences from two of the sporocyst morphotypes matched with adult trematodes from the intertidal fish: type 1 sporocyst was similar to Prosorhynchoides carvajali (Bucephalidae), with a mean genetic divergence of 0.78%, and type 2 sporocyst was similar to Proctoeces sp. (but not P. lintoni), with 0% genetic divergence. The third species (type 3 sporocyst) was classified to the family Fellodistomidae; however, the sequence from this species differed greatly from the three other fellodistomid species documented in the marine fish of Chile and from other fellodistomids in public databases. Moreover, this morphotype has a particular cercarial morphology that greatly differs from other fellodistomid species described thus far. Therefore, this intriguing trematode remains a mystery.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 3 , September 2013 , pp. 356 - 363
Copyright
Copyright © Cambridge University Press 2012 

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