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Monoamine oxidase in adult Ascaridia galli*

Published online by Cambridge University Press:  05 June 2009

S. K. Mishra ,
Ramanuj Sen  and
S. Ghatak
S. K. Mishra
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow–226001, India
Ramanuj Sen
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow–226001, India
S. Ghatak
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow–226001, India
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Abstract

Monoamine oxidase (MAO), catalysing oxidative deamination of biogenic monoamines, has been detected in adult Ascaridia galli. MAO was present in mitochondria and deaminated noradrenaline at the maximal rate, although serotonin, adrenaline, tyramine and dopamine were also degraded but more slowly. Of the organs studied, the body wall, female reproductive organ and intestine, the body wall (containing neuronal structures) showed highest MAO activity. Km value for chick ascarid mitochondrial MAO using tyramine as substrate was 1·66 x 10−3 M and it was most active at 2·5 mm tyramine concentration, pH 7·5 and 40°C. MAO of A. galli appeared to be thermolabile as nearly 80% of its activity was lost when the incubation temperature was increased 5° above optimum.

Type
Research Papers
Information
Journal of Helminthology , Volume 57 , Issue 4 , December 1983 , pp. 313 - 318
Copyright
Copyright © Cambridge University Press 1983

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