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Molecular identification of species of Taenia causing bovine cysticercosis in Ethiopia

Published online by Cambridge University Press:  04 March 2013

Z. Hailemariam ,
M. Nakao ,
S. Menkir ,
A. Lavikainen ,
T. Iwaki ,
T. Yanagida ,
M. Okamoto  and
A. Ito
Z. Hailemariam
Affiliation:
College of Veterinary Medicine, Haramaya University, PO Box 281, Dire Dawa, Ethiopia
M. Nakao*
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan
S. Menkir
Affiliation:
Department of Biology, Haramaya University, PO Box 138, Dire Dawa, Ethiopia
A. Lavikainen
Affiliation:
Infection Biology Program/Department of Bacteriology and Immunology, Haartman Institute, PO Box 21, FI-00014 University of Helsinki, Finland
T. Iwaki
Affiliation:
Meguro Parasitological Museum, Shimomeguro, Meguro-ku, Tokyo153-0064, Japan
T. Yanagida
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan
M. Okamoto
Affiliation:
Section of Wildlife Diversity, Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi484-8506, Japan
A. Ito
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan
*
*E-mail: [email protected]
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Abstract

Bovine cysticercosis causing damage to the beef industry is closely linked to human taeniasis due to Taenia saginata. In African countries, Taenia spp. from wildlife are also involved as possible sources of infections in livestock. To identify the aetiological agents of bovine cysticercosis in Ethiopia, cysticerci were collected from 41 cattle slaughtered in the eastern and central areas during 2010–2012. A single cysticercus per animal was subjected to the polymerase chain reaction (PCR)-based DNA sequencing of mitochondrial cytochrome c oxidase subunit 1 gene, and the resultant sequence was compared with those of members of the genus Taenia. Although 38 out of 41 cysticerci (92.7%) were identified as T. saginata, three samples (7.3%) showed the hitherto unknown sequences of Taenia sp., which is distantly related to Taenia solium, Taenia arctos and Taenia ovis. Old literatures suggest it to be Taenia hyaenae, but morphological identification of species could not be completed by observing only the larval samples.

Type
Short Communications
Information
Journal of Helminthology , Volume 88 , Issue 3 , September 2014 , pp. 376 - 380
Copyright
Copyright © Cambridge University Press 2013 

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References

Abunna, F., Tilahun, G., Megersa, B. & Regassa, A. (2007) Taeniasis and its socioeconomic implications in Awassa town and its surroundings, Southern Ethiopia. East African Journal of Public Health 4, 73–79.Google Scholar
Baer, J.G. (1926) Contributions on the helminth-fauna of South Africa. Reports of the Director of Veterinary Education and Research 11–12, pp. 63–136. Pretoria, Deparment of Agriculture, South Africa.Google Scholar
Bowles, J. & McManus, D.P. (1994) Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. American Journal of Tropical Medicine and Hygiene 50, 33–44.Google Scholar
Flisser, A., Correa, D., Avilla, G. & Marvilla, P. (2005) Biology of Taenia solium, Taenia saginata and Taenia saginata asiatica . pp. 1–8 in Murrell, K.D. (Ed.) WHO/FAO/OIE guidelines for the surveillance, prevention and control of taeniosis/cysticercosis. Paris, WHO.Google Scholar
Galimberti, A., Romano, D.F., Genchi, M., Paoloni, D., Vercillo, F., Bizzarri, L., Sassera, D., Bandi, C., Genchi, C., Ragni, B. & Casiraghi, M. (2012) Integrative taxonomy at work: DNA barcoding of taeniids harboured by wild and domestic cats. Molecular Ecology Resources 12, 403–413.Google Scholar
Gasser, R.B., Zhu, X. & McManus, D.P. (1999) NADH dehydrogenase subunit 1 and cytochrome c oxidase subunit I sequences compared for members of the genus Taenia (Cestoda). International Journal for Parasitology 29, 1965–1970.Google Scholar
Giesecke, W.H. (1997) Prevalence and economic implications of taeniasis/cysticercosis in South Africa. Report on a Workshop held at the Onderstepoort Veterinary Institute, Onderstepoort, South Africa, 18–19 August 1997, pp. 19–70.Google Scholar
Graber, M. (1978) À propos de la cysticercose musculaire des ruminants sauvages et domestiques d'Ethiopie. Revue d'élevage et de médecine vétérinaire des pays tropicaux 31, 33–37.Google Scholar
Hailemariam, Z., Nakao, M., Menkir, S., Lavikainen, A., Yanagida, T., Okamoto, M. & Ito, A. (2012) Molecular identification of unilocular hydatid cysts from domestic ungulates in Ethiopia: implications for human infections. Parasitology International 61, 375–377.Google Scholar
Haukisalmi, V., Lavikainen, A., Laaksonen, S. & Meri, S. (2011) Taenia arctos n. sp. (Cestoda: Cyclophyllidea: Taeniidae) from its definitive (brown bear Ursus arctos Linnaeus) and intermediate (moose/elk Alces spp.) hosts. Systematic Parasitology 80, 217–230.Google Scholar
Hoberg, E.P. (2006) Phylogeny of Taenia: species definitions and origins of human parasites. Parasitology International 55, S23–S30.Google Scholar
Hoberg, E.P., Alkire, N.L., de Queiroz, A. & Jones, A. (2001) Out of Africa: origins of the Taenia tapeworms in humans. Proceedings of the Royal Society B: Biological Sciences 268, 781–787.Google Scholar
Jia, W.Z., Yan, H.B., Guo, A.J., Zhu, X.Q., Wang, Y.C., Shi, W.G., Chen, H.T., Zhan, F., Zhang, S.H., Fu, B.Q., Littlewood, D.T. & Cai, X.P. (2010) Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics 11, 447.CrossRefGoogle Scholar
Jones, A. & Pybus, M.J. (2001) Taeniasis and echinococcosis. pp. 150–192 in Samuel, W.M., Pybus, M.J. & Kocan, A.A. (Eds) Parasitic diseases of wild mammals. 2nd edn. London, Manson Publishing.CrossRefGoogle Scholar
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16, 111–120.Google Scholar
Kumar, A. & Tadesse, G. (2011) Bovine cysticercosis in Ethiopia: a review. Ethiopian Veterinary Journal 15, 15–35.CrossRefGoogle Scholar
Lavikainen, A., Haukisalmi, V., Lehtinen, M.J., Henttonen, H., Oksanen, A. & Meri, S. (2008) A phylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology 135, 1457–1467.Google Scholar
Lavikainen, A., Laaksonen, S., Beckmen, K., Oksanen, A., Isomursu, M. & Meri, S. (2011) Molecular identification of Taenia spp. in wolves (Canis lupus), brown bears (Ursus arctos) and cervids from North Europe and Alaska. Parasitology International 60, 289–295.Google Scholar
Liu, G.H., Lin, R.Q., Li, M.W., Liu, W., Liu, Y., Yuan, Z.G., Song, H.Q., Zhao, G.H., Zhang, K.X. & Zhu, X.Q. (2011) The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans. Molecular Biology Reports 38, 2249–2256.Google Scholar
Loos-Frank, B. (2000) An up-date of Verster's (1969) 'Taxonomic revision of the genus Taenia Linnaeus' (Cestoda) in table format. Systematic Parasitology 45, 155–183.Google Scholar
Mettrick, D.F. (1963) Some trematodes and cestodes from mammals of Central Africa. Revista de Biologia Lisboa 3, 149–170.Google Scholar
Ministry of Agriculture (1972) Meat Inspection Regulations Legal notice No. 428 Negarite Gazexa. Addis Ababa, Ethiopia, Ministry of Agriculture.Google Scholar
Ortlepp, R.J. (1938) South African helminthes – Part II. Some Taenias from large wild carnivores. Onderstepoort Journal of Veterinary Science and Animal Industry 10, 253–278.Google Scholar
Regassa, A., Abunna, F., Mulugeta, A. & Megersa, B. (2009) Major metacestodes in cattle slaughtered at Wolaita Soddo Municipal abattoir, Southern Ethiopia: prevalence, cyst viability, organ distribution and socioeconomic implications. Tropical Animal Health and Production 41, 1495–1502.Google Scholar
Rossin, M.A., Timi, J.T. & Hoberg, E.P. (2010) An endemic Taenia from South America: validation of T. talicei Dollfus, 1960 (Cestoda: Taeniidae) with characterization of metacestodes and adults. Zootaxa 2636, 49–58.Google Scholar
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.Google Scholar
Verster, A. (1969) A taxonomic revision of the genus Taenia Linnaeus, 1758 s. str. Onderstepoort Journal of Veterinary Research 36, 3–58.Google ScholarPubMed
Yoder, D.R., Ebel, E.D., Hancock, D.D. & Combs, B.A. (1994) Epidemiologic findings from an outbreak of cysticercosis in feedlot cattle. Journal of the American Veterinary Medical Association 205, 45–50.Google Scholar
Zhang, L., Hu, M., Jones, A., Allsopp, B.A., Beveridge, I., Schindler, A.R. & Gasser, R.B. (2007) Characterization of Taenia madoquae and Taenia regis from carnivores in Kenya using genetic markers in nuclear and mitochondrial DNA, and their relationships with other selected taeniids. Molecular and Cellular Probes 21, 379–385.Google Scholar