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Molecular evidence shows that the liver fluke Fasciola gigantica is the predominant Fasciola species in ruminants from Pakistan

Published online by Cambridge University Press:  11 March 2015

U. Chaudhry ,
B. van Paridon ,
M.Z. Shabbir ,
M. Shafee ,
K. Ashraf ,
T. Yaqub  and
J. Gilleard
U. Chaudhry
Affiliation:
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Alberta, Canada
B. van Paridon*
Affiliation:
Department of Biology, Faculty of Science, University of Lethbridge, Alberta, Canada
M.Z. Shabbir
Affiliation:
Quality Operations Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan
M. Shafee
Affiliation:
Centre for Advanced Studies in Vaccine and Biotechnology, University of Baluchistan, Quetta, Pakistan
K. Ashraf
Affiliation:
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan
T. Yaqub
Affiliation:
Quality Operations Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan
J. Gilleard
Affiliation:
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Alberta, Canada
*
*E-mail: [email protected]
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Abstract

Fascioliasis is an important disease affecting livestock, with great costs to producers worldwide. It has also become a serious issue for human populations in some endemic areas as an emerging zoonotic infection. There are two Fasciola species of liver fluke responsible for this disease, which occur worldwide, Fasciola hepatica and Fasciola gigantica. Identifying these two species on the basis of adult or egg morphology requires specialist knowledge due to the similarity of characters, and may misidentify putative intermediate or hybrid forms. In this study we sequenced the internal transcribed spacer 2 (ITS-2) rDNA of liver flukes collected from multiple species of hosts from seven localities in the Punjab and Baluchistan provinces of Pakistan, to determine the distribution of these two species. All 46 flukes processed in this study, collected from seven sites, showed the rDNA ITS-2 genotype corresponding to F. gigantica, contradicting previous reports, based on adult and egg morphology, that both species are present in Pakistan, with F. hepatica being the more common.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 2 , March 2016 , pp. 206 - 213
Copyright
Copyright © Cambridge University Press 2015 

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