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Molecular evidence confirms that Proctoeces humboldti and Proctoeces chilensis (Digenea: Fellodistomidae) are the same species

Published online by Cambridge University Press:  15 January 2010

I.M. Valdivia ,
L. Cardenas ,
K. Gonzalez ,
D. Jofré ,
M. George-Nascimento ,
R. Guiñez  and
M.E. Oliva
I.M. Valdivia*
Affiliation:
Facultad de Recursos del Mar, Instituto de Investigaciones Oceanológicas, Universidad Antofagasta, Casilla 170, Antofagasta, Chile Programa de Doctorado en Ciencias Aplicadas, Mención en Sistemas Marinos Costeros, Universidad de Antofagasta, Antofagasta, Chile
L. Cardenas
Affiliation:
Instituto de Ecología y Evolución, Universidad Austral, Casilla 567, Isla Teja, Valdivia, Chile
K. Gonzalez
Affiliation:
Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Casilla 297, Concepción, Chile
D. Jofré
Affiliation:
Facultad de Recursos del Mar, Instituto de Investigaciones Oceanológicas, Universidad Antofagasta, Casilla 170, Antofagasta, Chile
M. George-Nascimento
Affiliation:
Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Casilla 297, Concepción, Chile
R. Guiñez
Affiliation:
Facultad de Recursos del Mar, Instituto de Investigaciones Oceanológicas, Universidad Antofagasta, Casilla 170, Antofagasta, Chile
M.E. Oliva
Affiliation:
Facultad de Recursos del Mar, Instituto de Investigaciones Oceanológicas, Universidad Antofagasta, Casilla 170, Antofagasta, Chile
*
*Fax: +56-55-637804 E-mail: [email protected]
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Abstract

Two species of Proctoeces Odhner, 1911 have been described in marine organisms from Chile: P. humboldti George-Nascimento & Quiroga (1983), parasitizing the gonads of keyhole limpets (Fissurella spp.), and P. chilensis Oliva (1984), an intestinal parasite of Sicyases sanguineus (Teleostei); both species were subsequently considered as P. lintoni Siddiqi & Cable (1960). To assist in the resolution of the taxonomic identification of Proctoeces species in marine organisms from Chile, phylogenetic studies using DNA sequences from the V4 region of the SSU rRNA gene were performed. Several specimens of P. lintoni were isolated from keyhole limpets (Fissurella spp.) and clingfish (S. sanguineus) from Bahia San Jorge (23°40′S) and Bahia Concepción (36°50′S). Phylogenetic analyses were conducted using three different approaches: a neighbour-joining (NJ), a maximum likelihood (ML) and Bayesian inference (BI). The phylogenetic analysis confirms that specimens of Proctoeces obtained from keyhole limpets and those specimens from the clingfish are in fact the same species. We prefer to consider our specimens as Proctoeces cf. lintoni, as the morphology of Proctoeces appears to be of doubtful value and genetic information about P. lintoni Siddiqi & Cable (1960) is not available. In addition, our results strongly suggest that there are at least three species in this genus.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 4 , December 2010 , pp. 341 - 347
Copyright
Copyright © Cambridge University Press 2010

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