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Molecular characterization of Neoechinorhynchus cylindratus Van Cleave, 1913 (Acanthocephala: Neoechinorhynchidae), a parasite of the largemouth bass (Micropterus salmoides) in northern Mexico

Published online by Cambridge University Press:  03 December 2018

M. García-Varela Open the ORCID record for M. García-Varela [Opens in a new window]  and
C.D. Pinacho-Pinacho
M. García-Varela*
Affiliation:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, CP 04510, Mexico City
C.D. Pinacho-Pinacho
Affiliation:
Instituto de Ecología, A.C. Red de Biología Evolutiva, km 2.5 Ant. Carretera a Coatepec, Xalapa, 91070 Veracruz, Mexico
*
Author for correspondence: M. García-Varela, E-mail: [email protected]
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Abstract

Members of the genus Neoechinorhynchus Stiles & Hassall, 1905 are endoparasites of freshwater fishes, brackish water fishes, and freshwater turtles distributed worldwide. In North America, 33 species have been described. One of the most widely distributed species in the eastern United States and Canada is Neoechinorhynchus (Neoechinorhynchus) cylindratus, a common acanthocephalan that infects centrarchid fishes. In the current study, adult specimens of N. (N) cylindratus were collected from largemouth bass (Micropterus salmoides) from the Purificación River in northern Mexico. In the same freshwater system, two additional congeneric species (Neoechinorhynchus (Neoechinorhynchus) emyditoides and Neoechinorhynchus (Neoechinorhynchus) panucensis) were collected and analysed. Sequences of the large subunit, internal transcribed spacers ITS1 and ITS2, 5.8S from nuclear DNA, and sequences of the cytochrome c oxidase subunit I (cox 1) from mitochondrial DNA were generated and aligned with other sequences obtained from GenBank. Maximum likelihood and Bayesian inference analyses inferred for each dataset showed that N. (N) panucensis, N. (N) emyditoides and N. (N) cylindratus were nested within several clades, indicating that these species do not share a common ancestor. Our phylogenies also revealed that the genus Neoechinorhynchus is paraphyletic, requiring further taxonomic revision using phylogenetic systematics and re-examination of morphological and ecological data. The presence of several N. (N) cylindratus adults in northern Mexico allowed us to typify this species for the first time using a combination of morphological and molecular characteristics. The current record shows a wide distribution range of N. (N) cylindratus across Canada, the United States and Mexico in the Nearctic region.

Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e18
Copyright
Copyright © Cambridge University Press 2018 

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References

Amin, OM (2002) Revision of Neoechinorhynchus Stiles and Hassall, 1905 (Acanthocephala: Neoechinorhynchidae) with keys to 88 species in two subgenera. Systematic Parasitology 53, 1–18.Google Scholar
Amin, OM (2013) Classification of the Acanthocephala. Folia Parasitologica 60, 273–305.Google Scholar
Amin, OM and Heckmann, R (2009) Description of Neoechinorhynchus (Neoechinorhynchus) buckneri n. sp. from the blacktailed redhorse Moxostoma poecilurum (Catostomidae) in the Tchoutacabouffa River, Mississippi, with a key to species of Neoechinorhynchus with different dorsoventral body wall thickness. Comparative Parasitology 76, 154–161.Google Scholar
Barger, MA, Thatcher, EV and Nickol, BB (2004) A new species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) from a red-eared slider (Trachemys scripta elegans) in Mexico. Comparative Parasitology 71, 1–3.Google Scholar
Bowles, J and McManus, DP (1993) Rapid discrimination of Echinococcus species and strains using a PCR-based method. Molecular and Biochemical Parasitology 57, 231–239.Google Scholar
Echi, PC et al. (2015) Mitochondrial DNA resolution of two new sequences Polyacanthorhynchus echiyensis n. sp. and Polyacanthorhynchus nigerianus n. sp. (Polyacanthocephala: Acanthocephala) in a parentenic host from a tropical river. Environment Conservation Journal 16, 13–17.Google Scholar
Galaviz, SL et al. (2016) Metazoan parasites of Micropterus salmoides (Centrarchidae: Perciformes) from reservoirs of Nuevo León, México and their association with condition factor and gender. Revista de Biología Tropical 64, 559–569.Google Scholar
García-Varela, M and Nadler, SA (2005) Phylogenetic relationships of Palaeacanthocephala (Acanthocephala) inferred from SSU and LSU rDNA gene sequences. Journal of Parasitology 91, 1401–1409.Google Scholar
García-Varela, M, García-Prieto, L and Pérez Rodríguez, R (2011) Molecular identification and first description of the male of Neoechinorhynchus schmidti (Acanthocephala: Neoechinorhynchidae), a parasite of Trachemys scripta (Testudines) in México. Parasitology International 60, 433–439.Google Scholar
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.Google Scholar
Malyarchuk, B et al. (2014) Phylogenetic relationships among Neoechinorhynchus species (Acanthocephala: Neoechinorhynchidae) from North-East Asia based on molecular data. Parasitology International 63, 100–107.Google Scholar
Martínez-Aquino, A et al. (2009) Detecting a complex of cryptic species within Neoechinorhynchus golvani (Acanthocephala: Neoechinorhynchidae) inferred from ITSs and LSU rDNA gene sequences. Journal of Parasitolology 95, 1040–1047.Google Scholar
McDonald, TE and Margolis, L (1995) Synopsis of the Parasites of Fishes of Canada: Supplement (1978–1993). Ottawa: NRC Research Press.Google Scholar
Muzzall, PM and Gillilland, MG (2004) Occurrence of acanthocephalans in largemouth bass and smallmouth bass (Centrarchidae) from Gull Lake, Michigan. Journal of Parasitology 90, 663–664.Google Scholar
Near, TJ, Garey, JR and Nadler, SA (1998) Phylogenetic relationships of the Acanthocephala inferred from 18S ribosomal DNA sequences. Molecular Phylogenetics and Evolution 10, 287–298.Google Scholar
Pinacho-Pinacho, CD, Pérez-Ponce de León, G and García-Varela, M (2012) Description of a new species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) a parasite of Dormitator latifrons from Southwestern Mexico based on morphological and molecular characters. Parasitology International 61, 634–644.Google Scholar
Pinacho-Pinacho, CD, Sereno-Uribe, AL and García-Varela, M (2014) Morphological and molecular data reveal a new species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) from Dormitator maculatus in the Gulf of Mexico. Parasitology International 63, 763–771.Google Scholar
Pinacho-Pinacho, CD et al. (2015) Checklist of the species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) in fishes and turtles in Middle-America, and their delimitation based on sequences of the 28S rDNA. Zootaxa 3985, 98–116.Google Scholar
Pinacho-Pinacho, CD et al. (2017) Mayarhynchus karlae n.g., n. sp. (Acanthocephala: Neoechinorhynchidae), a parasite of cichlids (Perciformes: Cichlidae) in southeastern Mexico, with comments on the paraphyly of Neoechynorhynchus Stiles & Hassall, 1905. Systematic Parasitology 94, 351–365.Google Scholar
Pinacho-Pinacho, CD et al. (2018) A hyper-diverse genus of acanthocephalans revealed by tree-base and non-tree based species delimitation methods: ten cryptic species of Neoechinorhynchus in Middle America freshwater fishes. Molecular Phylogenetics and Evolution 127, 30–45.Google Scholar
Richardson, DJ and Abdo, A (2011) Postcyclic transmission of Leptorhynchoides thecatus and Neoechinorhynchus cylindratus (Acanthocephala) to largemouth bass (Micropterus salmoides). Comparative Parasitology 78, 233–235.Google Scholar
Ronquist, F et al. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542.Google Scholar
Rosas-Valdez, R, Morrone, JJ and García-Varela, M (2012) Molecular phylogenetics of Floridosentis Ward, 1953 (Acanthocephala: Neoechinorhynchidae) parasites of mullets (Osteichthyes) from Mexico, using 28S rDNA sequences. Journal of Parasitology 98, 855–862.Google Scholar
Salgado-Maldonado, G (2013) Redescription of Neoechinorhynchus (Neoechinorhynchus) golvani Salgado-Maldonado, 1978 (Acanthocephala: Neoechinorhynchidae) and description of a new species from freshwater cichlids (Teleostei: Cichlidae) in Mexico. Parasitology Research 112, 1891–1901.Google Scholar
Smales, LR (2013) A review of the genus Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) from Australia with the description of two new species. Journal of Parasitology 99, 1106–1112.Google Scholar
Song, R et al. (2014) Population genetic structure of the Acanthocephalan Acanthosentis cheni in anadromous, freshwater, and landlocked stocks of its fish host, Coilia nasus. Journal of Parasitology 100, 193–197.Google Scholar
Van Cleave, HJ (1919) Acanthocephala from the Illinois River with descriptions of species and a synopsis of the family Neoechinorhynchidae. Bulletin of the Illinois National History Survey 13, 225–257.Google Scholar
Ward, HL (1940) Studies on the life history of Neoechinorhynchus cylindratus (Van Cleave, 1913) (Acanthocephala). Transactions of the American Microscopical Society 59, 327–347.Google Scholar