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Methods for quantifying eggs and oviposition rate of Dawestrema cycloancistrium (Monogenea: Dactylogyridae), monogenean parasite of Arapaima gigas (Teleostei: Osteoglossidae)

Published online by Cambridge University Press:  26 October 2018

P.O. Maciel Open the ORCID record for P.O. Maciel [Opens in a new window]  and
R.R. Alves Open the ORCID record for R.R. Alves [Opens in a new window]
P.O. Maciel*
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pesca e Aquicultura, Postal Box 90, 77008-900, Palmas, TO, Brazil
R.R. Alves
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pesca e Aquicultura, Postal Box 90, 77008-900, Palmas, TO, Brazil
*
Author for correspondence: P.O. Maciel, E-mail: [email protected]
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Abstract

Dawestrema cycloancistrium is the main ectoparasite causing mortality in fingerlings of pirarucu (Arapaima gigas) in Amazonian aquaculture. Very little is known about the D. cycloancistrium reproductive index and appropriate methods of collecting eggs for investigation. This study aimed to determine the oviposition rate of D. cycloancistrium. To achieve this aim, two egg quantification methods were tested: the estimative method (ME) and the total counting method (MT). Compared with the MT, the ME overestimated the number of eggs counted, which were 2943.5 ± 2840.6 and 1041.5 ± 533 eggs, and the oviposition rate, which was 80.1 ± 58.7 and 31.4 ± 16.4 eggs/parasite/day, for ME and MT, respectively. These results show that for studies quantifying D. cycloancistrium eggs, the total eggs in the sample must be counted, as the estimates made using subsamples are not representative. Using the MT, the oviposition rate for D. cycloancistrium was determined to be 31.4 ± 16.4 eggs per adult parasite per day. The present study demonstrates the egg production capacity of a monogenean species parasite of A. gigas, providing basic biological data for D. cycloancistrium.

Keywords

aquacultureDactylogyridaeegg collectionfish diseasegill parasiteMonogeneapaichepirarucu
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e4
Copyright
Copyright © Cambridge University Press 2018 

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References

Araújo, CSO et al. (2009) Parasitic infections in pirarucu fry, Arapaima gigas Schinz, 1822 (Arapaimatidae) kept in a semi-intensive fish farm in Central Amazon, Brazil. Veterinarski Arhiv 79, 499–507.Google Scholar
Bates, D et al. (2016) Fitting linear mixed-effects models using lme4. Journal of Statistical Software 67.Google Scholar
Buchmann, K (1988) Temperature-dependent reproduction and survival of Pseudodactylogyrus bini (Monogenea) on the European eel (Anguilla anguilla) Parasitology Research 75, 162–164.Google Scholar
Buchmann, K, Mellergaard, S and Køie, M (1987) Pseudodactylogyrus infections in eel: a review. Diseases of Aquatic Organisms 3, 51–57.Google Scholar
Buchmann, K, Uldal, A and Mellergaard, S (1994) Mortality of captive Arapaima gigas (Osteoglossidae) heavily infected with the gill monogenean Dawestrema cycloancistrium. Bulletin of the European Association of Fish Pathologists 14, 171–173.Google Scholar
Bush, AO et al. (1997) Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology 83, 557–583.Google Scholar
Cohen, SC, Justo, MCN and Kohn, A (2013) South American Monogenoidea Parasites of Fishes, Amphibians and Reptiles. Oficina de Livros, Rio de Janeiro, Brazil.Google Scholar
Delgado, PM et al. (2007) Ectoprasitological fauna in paiche Arapaima gigas (Schinz,1822) fingerlings cultured at the research center of Quistococha, Loreto, Peru. Folia Amazonica 16, 23–27.Google Scholar
Dinh Hoai, T and Hutson, KS (2014) Reproductive strategies of the insidious fish ectoparasite, Neobenedenia sp. (Capsalidae: Monogenea). PLoS ONE 9(9), e108801.Google Scholar
Gracenea, M et al. (2016) Diphasic sedimentation technique complemented by a statistical approach as a useful tool to the quantification and the determination of dynamics of eggs produced by adult monogenean parasitizing groupers, Epinephelus marginatus (Lowe, 1834), in captivity. Aquaculture Research 47, 830–837.Google Scholar
Imbiriba, EP (2001) Potencial de criação de pirarucu em cativeiro. Acta Amazonica 31, 299–316.Google Scholar
Kearn, GC (1986) The eggs of monogeneans. Advances in Parasitology 25, 175–273.Google Scholar
Kritsky, DC, Boeger, WA and Thatcher, VE (1985) Neotropical Monogenea. 7. Parasites of the pirarucu, Arapaima gigas (Cuvier), with descriptions of two new species and redescription of Dawestrema cycloancistrium Price and Nowlin, 1967 (Dactylogyridae: Ancyrocephalinae). Proceedings of the Biological Society of Washington 98, 321–331.Google Scholar
Lenth, RV (2016) Least-squares means: the R package lsmeans. Journal of Statistical Software 69, 1–33.Google Scholar
Lima, AF et al. (2015) Pirarucu culture in the Brazilian Amazon. Global Aquaculture Advocate, https://www.aquaculturealliance.org/advocate/pirarucu-culture-in-the-brazilian-amazon/.Google Scholar
Maciel, PO, Muniz, CR and Alves, RR (2017) Eggs hatching and oncomiracidia lifespan of Dawestrema cycloancistrium, a monogenean parasitic on Arapaima gigas. Veterinary Parasitology 247, 57–63.Google Scholar
Maciel, PO et al. (2018) Trichodinidae in commercial fish in South America. Reviews in Fish Biology and Fisheries 28, 33–56.Google Scholar
Marchiori, NC et al. (2015) Effect of water temperature and salinity in oviposition, hatching success and infestation of Aphanoblastella mastigatus (Monogenea, Dactylogyridae) on Rhamdia quelen. Brazilian Journal of Biology 75, 245–252.Google Scholar
Mooney, AJ, Ernst, I and Whittington, ID (2006) An egg-laying rhythm in Zeuxapta seriolae (Monogenea: Heteraxinidae), a gill parasite of yellowtail kingfish (Seriola lalandi). Aquaculture 253, 10–16.Google Scholar
Mooney, AJ, Ernst, I and Whittington, ID (2008) Egg-laying patterns and in vivo egg production in the monogenean parasites Heteraxine heterocerca and Benedenia seriolae from Japanese yellowtail Seriola quinqueradiata. Parasitology 135, 1295–1302.Google Scholar
Ogawa, K (2002) Impacts of diclidophorid monogenean infections on fisheries in Japan. International Journal for Parasitology 32, 373–380.Google Scholar
Ohno, Y, Kawano, F and Hirazawa, N (2008) Susceptibility by amberjack (Seriola dumerili), yellowtail (S. quinqueradiata) and Japanese flounder (Paralichthys olivaceus) to Neobenedenia girellae (Monogenea) infection and their acquired protection. Aquaculture 274, 30–35.Google Scholar
R Core Team (2018) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.Google Scholar
Rebellato-Junior Junior, IA et al. (2015) Reprodução e engorda do pirarucu: levantamento de processos produtivos e tecnologias. Embrapa, Brasília, Brazil.Google Scholar
Whittington, ID and Kearn, GC (2011) Hatching strategies in monogenean (Platyhelminth) parasites that facilitate host infection. Integrative and Comparative Biology 51, 91–99.Google Scholar