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Metacercariae of Heterodiplostomum lanceolatum (Trematoda: Proterodiplostomidae) found in Leptodactylus podicipinus (Anura: Leptodactylidae) from Brazil: a morphological, molecular and ecological study

Published online by Cambridge University Press:  23 July 2019

M.S. Queiroz Open the ORCID record for M.S. Queiroz [Opens in a new window] ,
D. López-Hernández ,
S.A. Locke ,
H.A. Pinto  and
L.A. Anjos
M.S. Queiroz*
Affiliation:
Universidade Estadual Paulista, Instituto de Biociências de Botucatu – IBB/Unesp, 18618-970, Botucatu, São Paulo, Brazil
D. López-Hernández
Affiliation:
Universidade Federal de Mina Gerais – UFMG, Belo Horizonte, Brazil
S.A. Locke
Affiliation:
Department of Biology, University of Puerto Rico at Mayagüez, Call Box 9000, Mayagüez, 00681-9000, Puerto Rico
H.A. Pinto
Affiliation:
Universidade Federal de Mina Gerais – UFMG, Belo Horizonte, Brazil
L.A. Anjos
Affiliation:
Departamento de Biologia e Zootecnia – FEIS/UNESP, Universidade Estadual Paulista, Passeio, CEP 15385000 Ilha Solteira, Brazil
*
Author for correspondence: M.S. Queiroz, E-mail: [email protected]
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Abstract

The trematodes from South American reptiles are poorly known, with only one life cycle completely characterized. We used molecular and morphological methods to characterize diplostomoid metacercariae found in 29 of 86 pointedbelly frogs, Leptodactylus podicipinus (Cope, 1862) collected in a marsh pond in Selvíria, in the central-west region of Brazil. The metacercariae were identified as Heterodiplostomum lanceolatum Dubois, 1936 (Proterodiplostomidae), a rarely reported species that matures in snakes. In phylogenetic analysis of partial sequences from 28S rDNA, H. lanceolatum fell within a polytomy with the proterodiplostomid Crocodilicola pseudostoma (molecular divergence of 4.1%) and other members of the superfamily Diplostomoidea. Our collections provide insights into the ecology of this parasite, in that infected frogs were smaller than uninfected frogs, and metacercariae were more numerous in the abdominal cavity and hindlimb muscles than in abdominal muscles, which suggests directions for future research on the transmission and pathology of this proterodiplostomid.

Keywords

Anuranslife cyclemetacercariaeparasitesTrematodaProterodiplostomidae
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e66
Copyright
Copyright © Cambridge University Press 2019 

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