Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-25T13:51:42.795Z Has data issue: false hasContentIssue false
  • English
  • Français

Light microscopic observations of Echinostoma caproni metacercariae during in vitro excystation

Published online by Cambridge University Press:  05 June 2009

R.L. Ursone  and
B. Fried
R.L. Ursone
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania, 18042, USA
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania, 18042, USA
*
* Author for correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Light microscopy was used to study excystation of Echinostoma caproni metacercariae in various media. Optimal excystation was observed in an alkaline trypsin-bile salts medium (TB) at 40 ± 1°C. The percentage of excystation in TB medium declined following either acid pepsin or acid saline pretreatment. Excystation rarely occurred in either trypsin or bile salts alone. Dissolution of the outer cyst occurred in TB medium thus facilitating excystation. Activation of the larva within the inner cyst led to its eventual release through an aperture in the mucoid plug region. Excystation of this species was facilitated by both intrinsic and extrinsic factors. There were considerable differences in excystation events between this species and the related allopatric species, E. trivolvis

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 3 , September 1995 , pp. 253 - 257
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Fried, B. & Roth, R.M. (1974) In vitro excystment of the metacercariae of Parorchis acanthus.Journal of Parasitology 60, 465.CrossRefGoogle ScholarPubMed
Fried, B., LeFlore, W.B. & Bass, H.S. (1984) Histochemical localization of hydrolytic enzymes in the cercariae and excysted metacercariae of Echinostorma revolutum (Trematoda). Proceedings of the Helminthologiail Society of Washington 51, 140–143.Google Scholar
Fried, B. & Emili, S. (1988) Excystation in vitro of Echinostoma liei and E. revolutum (Trematoda) metacercariae. Journal of Parasitology 74, 98–102.CrossRefGoogle Scholar
Fried, B. (1994) Metacercarial excystment of trematodes. Advances in Parasitology 33,91–144.CrossRefGoogle ScholarPubMed
Huffman, J.E. & Fried, B.(1990)Echinostoma and echinostomiasis. Advances in Parasitology 29,215–269.CrossRefGoogle ScholarPubMed
Smoluk, J. & Fried, B. (1994) Lightmicroscopic observations of Echinostorna trivolvis (Trematoda) metacercariae during in vitro excystation. Parasitology Research 80, 435–438.CrossRefGoogle Scholar