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Levels of Ancylostoma infections and phylogenetic analysis of cox 1 gene of A. ceylanicum in stray cat faecal samples from Guangzhou, China

Published online by Cambridge University Press:  30 June 2015

W. Hu ,
X.G. Yu ,
S. Wu ,
L.P. Tan ,
M.R. Song ,
A.Y. Abdulahi ,
Z. Wang ,
B. Jiang  and
G.Q. Li
W. Hu
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
X.G. Yu
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
S. Wu
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
L.P. Tan
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
M.R. Song
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
A.Y. Abdulahi
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
Z. Wang
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
B. Jiang
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
G.Q. Li*
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China
*
* E-mail: [email protected]
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Abstract

Ancylostoma ceylanicum is a common zoonotic nematode. Cats act as natural reservoirs of the hookworm and are involved in transmitting infection to humans, thus posing a potential risk to public health. The prevalence of feline A. ceylanicum in Guangzhou (South China) was surveyed by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). In total, 112 faecal samples were examined; 34.8% (39/112) and 43.8% (49/112) samples were positive with hookworms by microscopy and PCR method, respectively. Among them, 40.8% of samples harboured A. ceylanicum. Twelve positive A. ceylanicum samples were selected randomly and used for cox 1 sequence analysis. Sequencing results revealed that they had 97–99% similarity with A. ceylanicumcox 1 gene sequences deposited in GenBank. A phylogenetic tree showed that A. ceylanicum isolates were divided into two groups: one comprising four isolates from Guangzhou (South China), and the other comprising those from Malaysia, Cambodia and Guangzhou. In the latter group, all A. ceylanicum isolates from Guangzhou were clustered into a minor group again. The results indicate that the high prevalence of A. ceylanicum in stray cats in South China poses a potential risk of hookworm transmission from pet cats to humans, and that A. ceylanicum may be a species complex worldwide.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 4 , July 2016 , pp. 392 - 397
Copyright
Copyright © Cambridge University Press 2015 

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References

Baker, M.K., Lange, L., Verster, A. & van der Plaat, S. (1989) A survey of helminths in domestic cats in the Pretoria area of Transvaal, Republic of South Africa. Part 1: The prevalence and comparison of burdens of helminths in adult and juvenile cats. Journal of the South African Veterinary Association 60, 139–142.Google Scholar
Becker, A.C., Rohen, M., Epe, C. & Schnieder, T. (2012) Prevalence of endoparasites in stray and fostered dogs and cats in Northern Germany. Parasitology Research 111, 849–857.Google Scholar
Bowman, D.D., Montgomery, S.P., Zajac, A.M., Eberhard, M.L. & Kazacos, K.R. (2010) Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends in Parasitology 26, 162–167.CrossRefGoogle ScholarPubMed
Chen, J., Xu, M.J., Zhou, D.H., Song, H.Q., Wang, C.R. & Zhu, X.Q. (2012) Canine and feline parasitic zoonoses in China. Parasites & Vectors 5, 152.Google Scholar
Coggins, J.R. (1998) Effect of season, sex, and age on prevalence of parasitism in dogs from southwestern Wisconsin. Journal of the Helminthological Society of Washington 65, 219–224.Google Scholar
Conlan, J.V., Khamlome, B., Vongxay, K., Elliot, A., Pallant, L., Sripa, B., Blacksell, S.D., Fenwick, S. & Thompson, R.C.A. (2012) Soil-transmitted helminthiasis in Laos: a community wide cross-sectional study of humans and dogs in a mass drug administration environment. American Journal of Tropical Medicine and Hygiene 86, 624–634.CrossRefGoogle Scholar
Gates, M.C. & Nolan, T.J. (2009) Endoparasite prevalence and recurrence across different age groups of dogs and cats. Veterinary Parasitology 166, 153–158.Google Scholar
Henriksen, S.A. & Christensen, J.P. (1992) Demonstration of Isospora suis oocysts in faecal samples. Veterinary Record 131, 443–444.Google Scholar
Hu, M., Chilton, N.B., Zhu, X.Q. & Gasser, R.B. (2002) Single-strand conformation polymorphism-based analysis of mitochondrial cytochrome c oxidase subunit 1 reveals significant substructuring in hookworm populations. Electrophoresis 23, 27–34.Google Scholar
Inpankaew, T., Schaer, F., Dalsgaard, A., Khieu, V., Chimnoi, W., Chhoun, C., Sok, D., Marti, H., Muth, S., Odermatt, P. & Traub, R.J. (2014) High prevalence of Ancylostoma ceylanicum hookworm infections in humans, Cambodia. Emerging Infectious Diseases 20, 976–982.Google Scholar
Jiraanankul, V., Aphijirawat, W., Mungthin, M., Khositnithikul, R., Rangsin, R., Traub, R.J., Piyaraj, P., Naaglor, T., Taamasri, P. & Leelayoova, S. (2011) Incidence and risk factors of hookworm infection in a rural community of central Thailand. American Journal of Tropical Medicine and Hygiene 84, 594–598.Google Scholar
Little, S.E., Johnson, E.M., Lewis, D., Jaklitsch, R.P., Payton, M.E., Blagburn, B.L., Bowman, D.D., Moroff, S., Tams, T., Rich, L. & Aucoin, D. (2009) Prevalence of intestinal parasites in pet dogs in the United States. Veterinary Parasitology 166, 144–152.Google Scholar
Liu, Y.J., Zheng, G.C., Alsarakibi, M., Zhang, X.H., Hu, W., Lu, P.Y., Lin, L.Q., Tan, L.P., Luo, Q. & Li, G.Q. (2013) Molecular identification of Ancylostoma caninum isolated from cats in Southern China based on complete ITS sequence. Biomedical Research International doi:10.1155/2013/868050.Google Scholar
Liu, Y., Zheng, G., Alsarakibi, M., Zhang, X., Hu, W., Lin, L., Tan, L., Luo, Q., Lu, P. & Li, G. (2014) The zoonotic risk of Ancylostoma ceylanicum isolated from stray dogs and cats in Guangzhou, South China. Biomedical Research International doi:10.1155/2014/208759.Google Scholar
Miranda, R.R., Tennessen, J.A., Blouin, M.S. & Rabelo, E.M. (2008) Mitochondrial DNA variation of the dog hookworm Ancylostoma caninum in Brazilian populations. Veterinary Parasitology 151, 61–67.Google Scholar
Ngui, R., Lim, Y.A.L., Traub, R., Mahmud, R. & Mistam, M.S. (2012) Epidemiological and genetic data supporting the transmission of Ancylostoma ceylanicum among human and domestic animals. PLoS Neglected Tropical Diseases 6, e1552.Google Scholar
Ngui, R., Mahdy, M.A.K., Chua, K.H., Traub, R. & Lim, Y.A.L. (2013) Genetic characterization of the partial mitochondrial cytochrome oxidase c subunit I (cox 1) gene of the zoonotic parasitic nematode, Ancylostoma ceylanicum from humans, dogs and cats. Acta Tropica 128, 154–157.Google Scholar
Palmer, C.S., Traub, R.J., Robertson, I.D., Hobbs, R.P., Elliot, A., While, L., Rees, R. & Thompson, R.C.A. (2007) The veterinary and public health significance of hookworm in dogs and cats in Australia and the status of A. ceylanicum . Veterinary Parasitology 145, 304–313.Google Scholar
Prociv, P. & Croese, J. (1996) Human enteric infection with Ancylostoma caninum hookworms reappraised in the light of a ‘new′ zoonosis. Acta Tropica 62, 23–44.Google Scholar
Robben, S.R.M., le Nobel, W.E., Dopfer, D., Hendrikx, W.M.L., Boersema, J.H., Fransen, F. & Eysker, M.E. (2004) Infections with helminths and/or protozoa in cats in animal shelters in the Netherlands. Tijdschrift Voor diergeneeskunde 129, 2–6.Google Scholar
Savilla, T.M., Joy, J.E., May, J.D. & Somerville, C.C. (2011) Prevalence of dog intestinal nematode parasites in south central West Virginia, USA. Veterinary Parasitology 178, 115–120.Google Scholar
Schuster, R.K., Thomas, K., Sivakumar, S. & O'Donovan, D. (2009) The parasite fauna of stray domestic cats (Felis catus) in Dubai, United Arab Emirates. Parasitology Research 105, 125–134.Google Scholar
Speare, R., Latasi, F.F., Nelesone, T., Harmen, S., Melrose, W., Durrheim, D. & Heukelbach, J. (2006) Prevalence of soil transmitted nematodes on Nukufetau, a remote Pacific island in Tuvalu. BMC Infectious Diseases 6, 110.Google Scholar
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.Google Scholar
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL-X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.Google Scholar
Traub, R.J. (2013) Ancylostoma ceylanicum, a re-emerging but neglected parasitic zoonosis. International Journal for Parasitology 43, 1009–1015.Google Scholar
Traub, R.J., Inpankaew, T., Sutthikornchai, C., Sukthana, Y. & Thompson, R.C.A. (2008) PCR-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostorniasis caused by Ancylostoma ceylanicum in temple communities in Bangkok. Veterinary Parasitology 155, 67–73.Google Scholar
Visco, R.J., Corwin, R.M. & Selby, L.A. (1977) Effect of age and sex on the prevalence of intestinal parasitism in dogs. Journal of the American Veterinary Medical Association 170, 835–837.Google Scholar