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Kato–Katz thick smears as a DNA source of soil-transmitted helminths

Published online by Cambridge University Press:  15 November 2018

K.J.L. Monteiro ,
D.A. Calegar ,
F.A. Carvalho-Costa ,
L.H. Jaeger Open the ORCID record for L.H. Jaeger [Opens in a new window]  and
Amazonas Research Group
K.J.L. Monteiro
Affiliation:
Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Leônidas Deane, sala 308, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, Brazil
D.A. Calegar
Affiliation:
Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Leônidas Deane, sala 308, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, Brazil
F.A. Carvalho-Costa
Affiliation:
Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Leônidas Deane, sala 308, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, Brazil Escritório Técnico Regional - Fundação Oswaldo Cruz, Piauí. Avenida Odilon Araújo, 372, Teresina, Piauí, Brazil
L.H. Jaeger*
Affiliation:
Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Leônidas Deane, sala 308, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, Brazil
Amazonas Research Group
Affiliation:
Programa de Pós-Graduação em Medicina Tropical, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Arthur Neiva, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, Brazil
*
Author for correspondence: L.H. Jaeger, E-mail: [email protected]
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Abstract

Despite the reduction in the prevalence of soil-transmitted helminthiases in many regions of the world, morbidity rates remain high in some rural regions. The Kato–Katz technique is a simple, inexpensive and field-applicable tool commonly used for the diagnosis and worm-burden characterization of these infections. Molecular studies have revolutionized our understanding of the epidemiology and evolutionary genetics of parasites. In this study we recovered helminthic DNA from Kato–Katz slides (n = 93) prepared in 2011 in the Brazilian Amazon. We achieved DNA recovery by polymerase chain reaction (PCR) in 84% of cases for Ascaris sp. and 75% of cases for hookworms. The sequencing confirmed the specific species of the amplicons. The slides stored for a few years could be analysed using this methodology, allowing access to DNA from a large collection of samples. We must consider the Kato–Katz thick smears as a source of helminth DNA. This can significantly reduce logistical difficulties in the field in terms of obtaining, preserving, transporting and initial processing of samples.

Keywords

DNAhelminthKato–KatzPCRphylogenysoil-transmitted helminths
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e10
Copyright
Copyright © Cambridge University Press 2018 

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Footnotes

*

Current address: Laboratório de Células Tronco e Parasitologia Molecular, Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, José Lourenço Kelmer, s/n, São Pedro, Juiz de Fora, Brazil. Zip Code: 36036-900

Amazonas Research Group: D.C. Portela Camara, A.C. de Souza, A.T. de L. Barbosa, A.M. de A. Cabral, C.V.C. Guacho, D. de J. Hardoim, F.B. dos Santos, L.S. de Assunção, M.H. da Silva, M.M. Portilho, V.A. Marques, M.N. Bóia

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