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Initial fractionation of adult Schistosoma bovis antigen for diagnosis of infection in cattle

Published online by Cambridge University Press:  05 June 2009

W. P. H. Duffus ,
J. M. Preston  and
C. H. Staak
W. P. H. Duffus
Affiliation:
Veterinary Research Laboratories, P.O. Kabette, Kenya
J. M. Preston
Affiliation:
Veterinary Research Laboratories, P.O. Kabette, Kenya
C. H. Staak
Affiliation:
Veterinary Research Laboratories, P.O. Kabette, Kenya
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Abstract

Initial work on the fractionation of Schistosoma bovis adult worms is described. Both male and female worms were homogenized and sonicated before being passed through Sephadex G200. Five peaks were obtained with male fractionation (designated Ml to M5) and four with female (designated Fl to F4). Using the complement fixation test S. bovis antigen activity was detected primarily in peaks Ml and Fl. With the indirect haemagglutination and indirect haemagglutination inhibition tests S. bovis antigen activity was detected primarily in peaks Ml, M2, M3, Fl and F2.

Type
Research Article
Information
Journal of Helminthology , Volume 49 , Issue 1 , March 1975 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1975

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