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The influence of temperature and osmotic stress on the development and eclosion of hookworm eggs

Published online by Cambridge University Press:  18 November 2009

Bernard E. Matthews
Bernard E. Matthews
Affiliation:
Department of Zoology, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JT, Scotland, UK.
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Abstract

Time lapse video micro recording techniques have been used to investigate the development and eclosion of Ancylostoma ceylanicum and A. tubaeforme eggs under a range of temperature and osmotic conditions. Over the range 15 to 35°C increasing temperature resulted in decreased development time, the hyperbolic form of the curve being similar to that found by other workers. The adoption of a developmental parameter to mark the start of timing provided more reproducible results than previously reported. Osmotic stress in the range 0·600 mOsM/kg provided by a number of inorganic salts and organic sugars increased hatching time but did not greatly reduce the percentage that hatched. There was no correlation between the ionic composition of the solution and its effect on the eggs. Solutions of EDTA, carbonates and iodides markedly reduced hatching. Behavioural studies of the pre-hatched larvae suggested that the eggs were permeable and susceptible to osmotic and ionic changes early in their development and well before they were ready to hatch. No appreciable differences were found between the two species of hookworm investigated.

Type
Research Article
Information
Journal of Helminthology , Volume 59 , Issue 3 , September 1985 , pp. 217 - 224
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Belle, E. A. (1959) The effect of microenvironment on the free-living stages of Bunostomum trigono-cephalum. Canadian Journal of Zoology, 37, 289–298.CrossRefGoogle Scholar
Clarke, A. J. & Perry, R. N. (1977) Hatching of cyst nematodes. ematologica, 23, 350–368.Google Scholar
Clarke, A. J. & Perry, R. N. (1980) Egg-shell permeability and hatching of Ascaris suum. Parasitology, 80, 447–456.CrossRefGoogle ScholarPubMed
Crofton, H. D. & Whitlock, J. H. (1965) Ecology and biological plasticity of sheep nematodes. IV. The biological significance of temperature x time hatching curves for eggs of sheep nematodes. Cornell Veterinarian, 55, 263–274.Google Scholar
Croll, N. A. (1974) Necator americanus: Activity in the egg and the mechansim of hatching. Experimental Parasitology, 35, 80–85.Google Scholar
Cruickshank, C. N. D., Cooper, J. R. & Corran, M. B. (1959) A new tissue culture chamber. Experimental Cell Research, 16, 695–698.CrossRefGoogle ScholarPubMed
Doncaster, C. C. & Seymour, M. K. (1973) Exploration and site selection of penetration site by Tylenchida. Nematologica, 19, 137–145.Google Scholar
Doncaster, C. C. & Shepherd, A. M. (1967) The behaviour of second-stage Heterodera rostochiensis larvae leading to their emergence from the egg. Nematologica, 13, 476–478.CrossRefGoogle Scholar
Ehrenstein, G. & Von Schierenberg, E. (1980) Cell lineages and development of Caenorhabditis elegans and other nematodes. In: Nematodes as Biological Models, Vol. 1. Behavioural and Developmental Models, (editor Zuckerman, B. M.) pp. 1–71, Academic Press Inc.: New York and London.Google Scholar