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In vivo effect of sublethal concentrations of albendazole metabolites on the structure of the reproductive organs of Dictyocaulus viviparus

Published online by Cambridge University Press:  05 June 2009

A.M.M. Osman ,
D.E. Jacobs  and
J.M. Plummer
A.M.M. Osman
Affiliation:
Department of Pathology and Infectious Diseases and Department of Veterinary Basic Sciences, The Royal Veterinary College (University of London), North Mymms, Hatfield, Herts, AL9 7TA, UK
D.E. Jacobs
Affiliation:
Department of Pathology and Infectious Diseases and Department of Veterinary Basic Sciences, The Royal Veterinary College (University of London), North Mymms, Hatfield, Herts, AL9 7TA, UK
J.M. Plummer
Affiliation:
Department of Pathology and Infectious Diseases and Department of Veterinary Basic Sciences, The Royal Veterinary College (University of London), North Mymms, Hatfield, Herts, AL9 7TA, UK
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Abstract

Dictyocaulus viviparus were harvested from calves treated daily with albendazole at dose-rates sub-lethal to this parasite and from matched untreated calves. Male and female reproductive tracts from the worms were examined by light and transmission electron microscopy. A first description of the testis of this nematode is provided. Cell division in the germinal zone of both male and female worms was arrested by in vivo exposure to albendazole metabolites and the cytoplasmic threads connecting the germinal cells to the rachis disappeared. In male worms from treated calves, the rachis was absent from the growth zone and severe ultrastructural damage of spermatogonia, spermatocytes and spermatozoa was apparent. In female worms, the rachis remained intact in the growth zone, obvious abnormalities being confined to the germinal zone, where the cytoplasm of the oogonia was disrupted and few nuclei seen, and to the uteri and ovijector which contained only undifferentiated ova. The detrimental effects of albendazole metabolites on male and female worms were quickly reversed after withdrawal of medication.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 2 , June 1994 , pp. 161 - 166
Copyright
Copyright © Cambridge University Press 1994

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References

Basch, P.F. & Clemens, L.E. (1989) Schistosoma mansoni: reversible destruction of testes by procarbazine. Comparative Biochemistry and Physiology C. Comparative Pharmacology and Toxicology 93, 397–401.CrossRefGoogle ScholarPubMed
Delves, C.J., Howells, R.E. & Post, R.J. (1986) Gametogenesis and fertilisation in Dirofilaria immilis (Nematoda: Filaroidea). Parasitology 92, 181–197.CrossRefGoogle Scholar
Delatour, P., Benoit, E., Lechenet, J. & Besse, S. (1990) Pharmacokinetics in sheep and cattle of albendazole administered by an intraruminal slow release capsule. Research in Veterinary Science 48, 271–275.CrossRefGoogle ScholarPubMed
Foor, W.J. (1983a) Nematoda pp. 221–256 in Adiyodi, K.G. & Adiyodi, R.G. (Eds) Reproductive biology of invertebrates. vol 2: spermatogenesis and sperm function. Chichester, John Wiley and Sons Ltd.Google Scholar
Foor, W.J. (1933b) Nematoda. pp. 223–256in Adiyodi, K.G. & Adiyodi, R.G. (Eds) Reproductive biology of invertebrates. vol 1: oogenesis, oviposition and oosorption. Chichester, John Wiley and Sons Ltd.Google Scholar
Frantz, M., Zahner, H. & Benten, P. (1990) Fine structure alterations in female Brugia malayi and Litosomoides carinii after in vivo treatment with flubendazole. Parasitology Research 76, 401–405.CrossRefGoogle Scholar
Gibbons, L.M. & Khalil, L.F. (1988) A revision of the genus Dictyocaulus Railliet and Henry, 1907 (Nematoda: Trichostrongyloidea) with the description of D. africanus n.sp. from African artiodactylids. Journal of African Zoology 15, 59–60.Google Scholar
Guttekova, A. & Brunanska, M. (1988) The ultrastructure of Dictyocaulus vivparus 1. Germinal zone of the ovaries. Helminthologia 25, 235–243.Google Scholar
Jacobs, D.E., Fisher, M.A., Osman, A.M.M., Pilkington, J.G. & Jones, P.A. (1994) Low level albendazole treatment for the control of gastrointestinal and pulmonary nematodes in cattle. In preparationGoogle Scholar
Lacey, E., Brady, R.L., Prichard, R.K. & Watson, T.R. (1987) Comparison of inhibition of polymerisation of mammalian tubulin and helminth ovicidal activity by benzimidazole carbamates. Veterinary Parasitology 23, 105–119.CrossRefGoogle ScholarPubMed
McKellar, Q.A. & Scott, E.W. (1990) The benzimidazole anthelmintic agents: a review. Journal of Veterinary Pharmacology and Therapeutics 23, 223–247.CrossRefGoogle Scholar
Martin, P.J., Anderson, N. & Jarrett, R.G. (1985) Resistance to benzimidazole anthelmintics in field strains of Ostertagia and Nematodirus in sheep. Australian Veterinary Journal 62, 38–43.CrossRefGoogle ScholarPubMed
Oakley, G.A. (1980) The recovery of Dictyocaulus viviparus from bovine lungs by lung perfusion: a modification of Inderbitzen's method. Research in Veterinary Science 29, 395–396.CrossRefGoogle ScholarPubMed
Osman, A.M.M. (1993) Studies of the effect of low level albendazole therapy in calves on Dictyocaulus viviparus (Railliet and Henry 1907). PhD thesis, University of London.Google Scholar
Prichard, R.K., Steel, J.W. & Hennessy, D.R. (1978) Prolonged administration: A new concept for increasing the spectrum and effectiveness of anthelmintics. Veterinary Parasitology 4, 309–315.CrossRefGoogle Scholar
Scott, E.W., Baxter, P. & Armour, J. (1991) Fecundity of anthelmintic resistant adult Haemonchus contortus after exposure to ivermectin or benzimidazoles in vivo. Research in Veterinary Science 50, 247–249.CrossRefGoogle ScholarPubMed
Shaw, M.K. & Erasmus, D.A. (1988) Schistosoma mansoni: praziquantel-induced changes to the female reproductive system. Experimental Parasitology 65, 31–42.CrossRefGoogle Scholar