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In vitro schistosomicidal effects of aqueous and dichloromethane fractions from leaves and stems of Piper species and the isolation of an active amide from P. amalago L. (Piperaceae)

Published online by Cambridge University Press:  08 April 2013

V.S. Carrara ,
S.C.H. Vieira ,
R.G. de Paula ,
V. Rodrigues ,
L.G. Magalhães ,
D.A.G. Cortez  and
A.A. Da Silva Filho
V.S. Carrara
Affiliation:
Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, PR, Brazil
S.C.H. Vieira
Affiliation:
Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, PR, Brazil
R.G. de Paula
Affiliation:
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
V. Rodrigues
Affiliation:
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
L.G. Magalhães
Affiliation:
Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil
D.A.G. Cortez
Affiliation:
Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, PR, Brazil
A.A. Da Silva Filho*
Affiliation:
Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil
*
*E-mail: [email protected]
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Abstract

Dichloromethane and aqueous fractions from leaves and stems of Piper arboreum Aubl., P. aduncum L., P. amalago L., P. crassinervium H.B. & K., P. diospyrifolium Kunth, P. hispidum Sw. and P. xylosteoides (Kunth) Steud. were tested against adult worms of Schistosoma mansoni. The in vitro activity was evaluated in terms of mortality, number of separated worms and number of worms with reduced motor activity. Most dichloromethane fractions from all Piper species showed moderate schistosomicidal activity, but aqueous fractions were not active. The dichloromethane fraction of P. amalago leaves (at 100 μg/ml) showed the highest activity, resulting in worm mortality, the separation of worm pairs and reduced motor activity. Chromatographic fractionation of the dichloromethane fraction of P. amalago leaves led to the isolation of its major compound, which was also tested against adults of S. mansoni. The isolated piperamide N-[7-(3′,4′-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl] pyrrolidine, at 100 μm, resulted in the mortality of all adult worms after 24 h of incubation. The findings suggest that species of Piper are potential sources of schistosomicidal compounds.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 3 , September 2014 , pp. 321 - 326
Copyright
Copyright © Cambridge University Press 2013 

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