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In vitro excystment of the metacercaria of Bucephalus haimeanus from the common goby (Pomatoschistus microps)

Published online by Cambridge University Press:  05 June 2009

H. El-Mayas  and
G.C. Kearn
H. El-Mayas
Affiliation:
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
G.C. Kearn
Affiliation:
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
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Abstract

The structure of the metacercarial cyst wall and excystment in vitro were studied in the bucephalid digenean Bucephalus haimeanus. Optimum excystment occurred in a mixture of 1% trypsin and 1% bile salts at pH 7.8, following a 15 min pretreatment with 1% pepsin at pH 2.0. Enzymes (pepsin, trypsin) facilitated excystment, but were not essential, since some of the active metacercariae perforated the cyst wall in their absence. Other distinctive features were the weak development of the cyst wall, the occurrence of some excystment in strongly acidic media, ability to survive for at least 2 h in acidic media (pH 2.0) and total inactivity and failure to excyst in bile salts. The possible significance of these features is considered in relation to the location of the cysts in the liver of the second intermediate host, the common goby (Pomatoschistus microps), and conditions experienced by the cysts when the goby is eaten by the definitive host, the bass (Morone labrax). Excystment occurred readily at temperatures from 10 to 40°C, but percentage and speed of excystment were reduced at 5°C.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 3 , September 1996 , pp. 193 - 200
Copyright
Copyright © Cambridge University Press 1996

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References

Asanji, M.F. & Williams, M.O. (1975) Studies on the excystment of trematode metacercariae in vitro. Zeitschrift für Parasitenkunde 47, 151–163.CrossRefGoogle ScholarPubMed
Barnabé, B. (1976) Biologie du loup Dicentrarchus labrax (L). Thèse de Doctorat. Université des Sciences et Techniques du Languedoc, Montpellier, France.Google Scholar
Chakrabarti, K.K. & Baugh, S.C. (1974) On the bucephalid metacercaria Bucephalopsis linguiformis n.sp. from Indian freshwater fishes Esomus danricus and Nemachilus botia. Netherlands Journal of Zoology 24, 50–57.Google Scholar
Chung, Y., Kong, Y., Joo, I., Cho, S. & Kang, S. (1995) Excystment of Paragonimus westermani metacercariae by endogenous cysteine protease. Journal of Parasitology 81, 137–142.CrossRefGoogle ScholarPubMed
Dixon, K.E. (1966) The physiology of excystment of the metacercaria of Fasciola hepatica L. Parasitology 56, 431–456.CrossRefGoogle ScholarPubMed
El-Mayas, H. & Kearn, G.C. (1995) In vitro excystment of the metacercaria of Cryptocotyle concavum from the common goby Pomatoschistus microps. Journal of Helminthology 69, 285–297.CrossRefGoogle Scholar
Fänge, R. & Grove, D. (1979) Digestion, pp. 161–260in Hoar, W.S., Randall, D.J. & Brett, J.R. (Eds) Fish physiology. London, Academic Press.Google Scholar
Fried, B., LeFlore, W.B. & Bass, H.S. (1984) Histochemical localization of hydrolytic enzymes in the cercaria and excysted metacercaria of Echinostoma revolutum (Trematoda). Proceedings of the Helminthological Society of Washington 51, 140–143.Google Scholar
Halton, D.W. & Johnston, B.R. (1982) Functional morphology of the metacercarial cysts of Bucephaloides gracilescens (Trematoda: Bucephalidae). Parasitology 85, 45–52.CrossRefGoogle Scholar
Higgins, J.C. (1980) Formation of the cyst wall and related changes in the structure of the tegument of Bucephalus haimeanus (Lacaze-Duthiers, 1854) during the metamorphosis from the cercarial to the metacercarial stage. Parasitology 81, 47–59.CrossRefGoogle Scholar
Higgins, J.C., Wright, D.E. & Matthews, R.A. (1977) Ultrastructure and histochemistry of the cyst wall of Bucephalus haimeanus (Lacaze-Duthiers, 1854). Parasitology 75, 207–214.CrossRefGoogle Scholar
Howell, M.J. (1970) Excystment of the metacercaria of Echinoparyphium serratum (Trematoda: Echinostomatidae). Journal of Helminthology 44, 35–56.CrossRefGoogle ScholarPubMed
Hsu, H.F. & Wang, L.S. (1938) Studies on certain problems of Clonorchis sinensis. IV. Notes on the resistance of cysts in fish flesh, the migration route and the morphology of the young worms in the final host. Chinese Medical Journal Supplement 2, 385–400.Google Scholar
Hunter, W.S. & Chait, D.C. (1952) Notes on the excystment and culture in vitro of the microphallid trematode, Gynaecotyla adunca (Linton, 1905). Journal of Parasitology 38, 87.CrossRefGoogle Scholar
Johnston, B.R. & Halton, D.W. (1981) Excystation in vitro of Bucephaloides gracilescens metacercaria (Trematoda: Bucephalidae). Zeitschrift für Parasitenkunde 65, 71–78.CrossRefGoogle Scholar
Kirschner, K. & Bacha, W.J. (1980) Excystment of Himasthla quissetensis (Trematoda: Echinostomatidae) metacercariae in vitro. Journal of Parasitology 66, 263–267.CrossRefGoogle Scholar
Lackie, A.M. (1975) The activation of infective stages of endoparasites of vertebrates. Biological Reviews 50, 285–323.CrossRefGoogle ScholarPubMed
Macy, R.W., Berntzen, A.K. & Benz, M. (1968) In vitro excystation of Sphaeridiotrema globulus metacercariae, structure of cyst, and the relationship to host-specificity. Journal of Parasitology 54, 28–38.CrossRefGoogle ScholarPubMed
Maillard, C. (1976) Distomatoses de poissons en milieu lagunaire. Thèse de Doctorat. Université des Sciences et Techniques du Languedoc, Montpellier, France.Google Scholar
Maillard, C. & Saad-Farès, A. (1981) Bucephalus baeri n. sp. parasite de Dicentrarchus labrax (Teleostei): description et cycle évolurif. Zeitschrift für Parasitenkunde 66, 31–40.CrossRefGoogle Scholar
Matthews, R.A. (1973) The life cycle of Bucephalus haimeanus Lacaze-Duthiers, 1854 from Cardium edule L. Parasitology 67, 341–350.CrossRefGoogle ScholarPubMed
Pandey, K.C. (1969) Bucephalopsis hexaglandulata sp.n., B. oxygasteri sp.n., and B. multiglandulata sp.n.: gasterostome metacercariae from Indian fishes. Transactions of the American Microscopical Society 88, 486–491.CrossRefGoogle Scholar
Schroeder, D.J., Johnson, A.D. & Mohammad, K.H. (1981) In vitro excystment of the blackspot trematode Neascus pyriformis Chandler, 1951 (Trematoda: Diplostomatidae). Proceedings of the Helminthological Society of Washington 48, 184–189.Google Scholar
Silverman, P.H. (1954) Studies on the biology of some tapeworms of the genus Taenia. I - Factors affecting hatching and activation of taeniid ova, and some criteria of their viability. Annals of Tropical Medicine and Parasitology 48, 207–216.CrossRefGoogle Scholar
Spellman, S.J. & Johnson, A.D. (1987) In vitro excystment of the blackspot trematode Uvulifer ambloplitis (Trematoda: Diplostomatidae). International Journal for Parasitology 17, 897–902.CrossRefGoogle Scholar