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In vitro culture of tetrathyridia of Mesocestoides corti using a gel based diphasic medium

Published online by Cambridge University Press:  12 April 2024

J. Chernin ,
D. Thompson  and
D. Swaine
J. Chernin*
Affiliation:
School of Biological Sciences, University of Portsmouth, King Henry Building, Portsmouth, PO1 2DY, Hants, UK
D. Thompson
Affiliation:
Research and Development, Oxoid Ltd, Wade Road, Basingstoke, RG24 8PW, Hants, UK
D. Swaine
Affiliation:
Research and Development, Oxoid Ltd, Wade Road, Basingstoke, RG24 8PW, Hants, UK
*
*Fax: 0293 92 842070 Email: [email protected]
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Abstract

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Tetrathyridia of Mesocestoides corti were cultured in vitro in a diphasic medium consisting of a liquid medium (CMRL Sigma) and a thixotropic nutrient gel (Oxoid). Tests demonstrated that a 50% medium/gel mixture produced optimum conditions for the survival and development of tetrathyridia. Established anthelminthic drugs were inoculated into the gel which demonstrated that this system can be used for preliminary anthelminthic drug screening. The development and survival of the tetrathyridia were influenced by the addition of pepsin, trypsin and liver peptone to the culture media. The development and maturation of proglottids were observed in addition to asexual reproduction by the process of budding. Tetrathyridia maintained in vitro and reinfected into both mouse and rat hosts retained their viability.

Type
Review Article
Information
Journal of Helminthology , Volume 76 , Issue 1 , March 2002 , pp. 21 - 25
Copyright
Copyright © Cambridge University Press 2002

References

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