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In vitro biological control of bovine parasitic nematodes by Arthrobotrys cladodes, Duddingtonia flagrans and Pochonia chlamydosporia under different temperature conditions

Published online by Cambridge University Press:  21 September 2020

Ítalo Stoupa Vieira Open the ORCID record for Ítalo Stoupa Vieira [Opens in a new window] ,
Isabela de Castro Oliveira ,
Artur Kanadani Campos  and
Jackson Victor de Araújo
Ítalo Stoupa Vieira*
Affiliation:
Laboratório de Parasitologia e Doenças Parasitárias, Departamento de Veterinária, Universidade Federal de Viçosa, Av. P.H Rolfs, s/n, Viçosa, Minas GeraisCEP 36570-900, Brazil
Isabela de Castro Oliveira
Affiliation:
Laboratório de Parasitologia e Doenças Parasitárias, Departamento de Veterinária, Universidade Federal de Viçosa, Av. P.H Rolfs, s/n, Viçosa, Minas GeraisCEP 36570-900, Brazil
Artur Kanadani Campos
Affiliation:
Laboratório de Parasitologia e Doenças Parasitárias, Departamento de Veterinária, Universidade Federal de Viçosa, Av. P.H Rolfs, s/n, Viçosa, Minas GeraisCEP 36570-900, Brazil
Jackson Victor de Araújo
Affiliation:
Laboratório de Parasitologia e Doenças Parasitárias, Departamento de Veterinária, Universidade Federal de Viçosa, Av. P.H Rolfs, s/n, Viçosa, Minas GeraisCEP 36570-900, Brazil
*
Author for correspondence: Ítalo Stoupa Vieira, E-mail: [email protected]
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Abstract

Variations in temperature can affect the development of nematophagous fungi, especially when they are used in the biological control of parasitic nematodes in the pastures where cattle are reared. The aim of this work was to evaluate the effects of temperature on the performance of nematophagous fungi in the biological control of bovine parasitic nematodes. The mycelial growth, chlamydospore production and nematicidal activity of Duddingtonia flagrans, Arthrobotrys cladodes and Pochonia chlamydosporia were evaluated at 15, 20, 25, 30 and 35°C. The fungal strains achieved mycelial growth, chlamydospore production and nematicidal activity on parasitic nematodes under all temperature conditions tested. The fungi showed higher growth at intermediate temperatures (20, 25 and 30°C) than at the extremes of 15 and 35°C. At 25 and 30°C, D. flagrans realized 96.8 and 94.5% nematicidal activity on bovine parasitic nematodes, respectively. Arthrobotrys cladodes effected nematicidal activity of 85.3 and 83.5%, at 20 and 25°C, respectively. At 20 and 30°C, P. chlamydosporia achieved nematicidal activity of 81.3 and 87.4%, respectively. The maximum chlamydospore production was reached at 20, 25 and 30°C for D. flagrans, at 20 and 25°C for A. cladodes and P. chlamydosporia. The results of this study demonstrated that the tested fungal strains of D. flagrans, A. cladodes and P. chlamydosporia, when used in the biological control of bovine parasitic nematodes, were not limited by in vitro temperature variations. Therefore, the use of these strains of fungi as biological control agents of parasitic nematodes is promising.

Keywords

Duddingtonia flagransArthrobotrys cladodesPochonia chlamydosporiatemperaturebiological controlnematodes
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e194
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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