Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-05T02:05:24.490Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro assessment of praziquantel and a novel nanomaterial against protoscoleces of Echinococcus granulosus

Published online by Cambridge University Press:  31 January 2011

S. De ,
D. Pan ,
A.K. Bera ,
V. Sreevatsava ,
S. Bandyopadhyay ,
D. Chaudhuri ,
S. Kumar ,
T. Rana ,
S. Das  and
S.K. Das
...Show all authors
S. De
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
D. Pan
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
A.K. Bera
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
V. Sreevatsava
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
S. Bandyopadhyay
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India NRC on Yak, Dirang, Arunachal Pradesh, India
D. Chaudhuri
Affiliation:
Central Avian Research Institute, Izatnagar, UP243 122, India
S. Kumar
Affiliation:
ARIS Cell, Indian Veterinary Research Institute, Izatnagar243 122, India
T. Rana
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
S. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
S.K. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
V.V. Suryanaryana
Affiliation:
FMD Research Laboratory, Indian Veterinary Research Institute, Bangalore560 024, India
M. Norjit Singh
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
D. Bhattacharya*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata700037, West Bengal, India
*
*Fax: +913325565725 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The present study describes the activity of a nanomaterial on protoscoleces of Echinococcus granulosus, which exhibited morphological changes and apoptosis. Apoptotic changes were deduced on the basis of effector caspase activation and nucleosomal laddering. Invaginated protoscoleces maintained in vitro became evaginated and had hooks, presumptive suckers and stalks. Degenerative changes of protoscoleces were evidenced after treatment with praziquantel and nano-combination. Protoscoleces treated with praziquantel had distinct attestation of necrosis and nano-combination-treated protoscoleces had signatures of apoptosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 1 , March 2012 , pp. 26 - 29
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the priciple of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Cryns, V. & Yuan, J. (1998) Proteases to die for. Genes and Development 12, 1551–1570.CrossRefGoogle Scholar
Lazebnik, Y.A., Cole, S., Cooke, C.A., Nelson, W.G. & Earnshaw, W.C. (1993) Nuclear events of apoptosis in vitro in cell-free mitotic extracts: a model system for analysis of the active phase of apoptosis. Journal of Cell Biology 123, 7–22.CrossRefGoogle Scholar
Liance, M., Nemati, F., Bories, C. & Couvreur, P. (1993) Experience with doxorubicin-bound polyisohexylcyanoacrylate nanoparticles on murine alveolar echinococcosis of the liver. International Journal for Parasitology 23, 427–429.CrossRefGoogle ScholarPubMed
Martin, S.J., O'Brien, G.A., Nishioka, W.K., McGahon, A.J., Mahboubi, A., Saido, T.C. & Green, D.R. (1995) Proteolysis of fodrin (non-erythroid spectrin) during apoptosis. Journal of Biological Chemistry 270, 6425–6428.CrossRefGoogle ScholarPubMed
Morris, D.L. & Taylor, D.H. (1988) Optimal timing of post-operative albendazole prophylaxis in E. granulosus. Annals of Tropical Medicine and Parasitology 82, 65–66.CrossRefGoogle ScholarPubMed
Morris, D.L., Richards, K.S. & Chinnery, J.B. (1986) Protoscolicidal effect of praziquantel – in vitro and electron microscopical studies on Echinococcus granulosus. Journal of Antimicrobial Chemotherapy 18, 687–691.CrossRefGoogle ScholarPubMed
Morris, D.L., Taylor, D.H., Daniels, D., Riley, E.M. & Richards, K.S. (1988) Determination of the minimum time of praziquantel therapy required for the in vitro treatment of protoscoleces of Echinococcus granulosus. Journal of Helminthology 62, 10–14.CrossRefGoogle ScholarPubMed
Naguleswaran, A., Spicher, M., Vonlaufen, N., Ortega-Mora, L.M., Torgerson, P., Gottstein, B. & Hemphill, A. (2006) In vitro metacestodicidal activities of genistein and other isoflavones against Echinococcus multilocularis and Echinococcus granulosus. Antimicrobial Agents and Chemotherapy 50, 3770–3778.CrossRefGoogle ScholarPubMed
Porter, A.G. & Janicke, R.U. (1999) Emerging roles of caspase-3 in apoptosis. Cell Death and Differentiation 6, 99–104.CrossRefGoogle ScholarPubMed
Rodrigues, J.M. Jr, Bories, C., Emery, I., Fessi, H., Devissaguet, J.P. & Liance, M. (1995) Development of an injectable formulation of albendazole and in vivo evaluation of its efficacy against Echinococcus multilocularis metacestode. International Journal for Parasitology 25, 1437–1441.CrossRefGoogle ScholarPubMed
Urrea-París, M.A., Casado, N., Moreno, M.J. & Rodriguez-Caabeiro, F. (2001) Chemoprophylactic praziquantel treatment in experimental hydatidosis. Parasitology Research 87, 510.Google ScholarPubMed
Walker, M., Rossignol, J.F., Torgerson, P. & Hemphill, A. (2004) In vitro effects of nitazoxanide on Echinococcus granulosus protoscoleces and metacestodes. Journal of Antimicrobial Chemotherapy 54, 609–616.CrossRefGoogle ScholarPubMed
Zhivotovsky, B. & Orrenius, S. (2001) Assesment of apoptosis and necrosis by DNA fragmentation and morphological criteria. pp. 18.3.1–18.3.23 in Bonofavino, J.S. (Ed.) Current protocols in cell biology. New York, John Wiley & Sons.Google Scholar