Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-17T13:57:46.987Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunohistochemical observation of local inflammatory cell infiltration in the host-tissue reaction site of human hydatid cysts

Published online by Cambridge University Press:  30 April 2018

R. Jafari ,
B. Sanei ,
A. Baradaran ,
M. Kolahdouzan ,
B. Bagherpour  and
H. Yousofi Darani
R. Jafari
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
B. Sanei
Affiliation:
Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
A. Baradaran
Affiliation:
Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
M. Kolahdouzan
Affiliation:
Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
B. Bagherpour
Affiliation:
Acquired Immunodeficiency Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
H. Yousofi Darani*
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
*
Author for correspondence: H. Yousofi Darani, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. The results were then compared to hepatocellular carcinoma and chronic hepatitis. In the host-tissue reaction site of liver hydatid cysts, a distinct pattern of local immune cell response, which outwardly consisted of a pack of the fibrous elements, a layer of palisading macrophages, an eosinophil-containing layer and a layer of accumulated lymphocytes, was observed. However, in some cases there were no positive cells for CD56+ natural killer cells and Foxp3+ regulatory T cells. The CD3+ T cells were the predominant inflammatory cells in all groups, followed by CD19+ B cells. It can be concluded that different immune cells are involved in the local response to human hydatid cysts.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 3 , May 2019 , pp. 277 - 285
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Current address: Infectious Disease and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

References

Craig, PS, McManus, DP, Lightowlers, MW et al. (2007) Prevention and control of cystic echinococcosis. The Lancet Infectious Diseases 7, 385–394.Google Scholar
Darani, HY and Yousefi, M (2012) Parasites and cancers: parasite antigens as possible targets for cancer immunotherapy. Future Oncology 8, 1529–1535.Google Scholar
Diaz, A, Willis, AC and Sim, RB (2000) Expression of the proteinase specialized in bone resorption, cathepsin K, in granulomatous inflammation. Molecular Medicine 6, 648–659.Google Scholar
Díaz, A, Casaravilla, C, Allen, JE, Sim, RB and Ferreira, AM (2011a) Understanding the laminated layer of larval Echinococcus II: immunology. Trends in Parasitology 27, 264–273.Google Scholar
Díaz, A, Casaravilla, C, Irigoín, F, Lin, G, Previato, JO and Ferreira, F (2011b) Understanding the laminated layer of larval Echinococcus I: structure. Trends in Parasitology 27, 204–213.Google Scholar
Frider, B, Larrieu, E and Odriozola, M (1999) Long-term outcome of asymptomatic liver hydatidosis. Journal of Hepatology 30, 228–231.Google Scholar
Gliwiński, M, Iwaszkiewicz-Grześ, D and Trzonkowski, P (2017) Cell-based therapies with T regulatory cells. BioDrugs 31, 335–347.Google Scholar
Hamilos, DL (1989) Antigen presenting cells. Immunologic Research 8, 98–117.Google Scholar
Kharebov, A, Nahmias, J and El-On, J (1997) Cellular and humoral immune responses of hydatidosis patients to Echinococcus granulosus purified antigens. The American Journal of Tropical Medicine and Hygiene 57, 619–624.Google Scholar
LeBien, TW and Tedder, TF (2008) B lymphocytes: how they develop and function. Blood 112, 1570–1580.Google Scholar
Lightowlers, MW (2010) Fact or hypothesis: concomitant immunity in taeniid cestode infections. Parasite Immunology 32, 582–589.Google Scholar
McManus, DP (2013) Current status of the genetics and molecular taxonomy of Echinococcus species. Parasitology 140, 1617–1623.Google Scholar
Mourglia-Ettlin, G, Marqués, JM, Chabalgoity, JA and Dematteis, S (2011) Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. PLoS Neglected Tropical Diseases 5(8), e1293. doi: 10.1371/journal.pntd.0001293.Google Scholar
Pakala, T, Molina, M and Wu, GY (2016) Hepatic echinococcal cysts: a review. Journal of Clinical and Translational Hepatology 4, 39–46.Google Scholar
Phuah, JY, Mattila, JT, Lin, PL and Flynn, JL (2012) Activated B cells in the granulomas of non-human primates infected with Mycobacterium tuberculosis. The American Journal of Pathology 181, 508–514.Google Scholar
Rickard, MD and Williams, JF (1982) Hydatidosis/cysticercosis: immune mechanisms and immunization against infection. Advances in Parasitology 21, 229–296.Google Scholar
Riganò, R, Profumo, E, Buttari, B, Delunardo, F, Ortona, E, Margutti, P, Teggi, A and Siracusano, A (2004) Cytokine expression in the follow-up of patients with cystic echinococcosis. Parassitologia 46, 441–442.Google Scholar
Rinaldi, L, Maurelli, MP, Veneziano, V, Capuano, F, Perugini, AG and Cringoli, S (2008) The role of cattle in the epidemiology of Echinococcus granulosus in an endemic area of southern Italy. Parasitology Research 103, 175–179.Google Scholar
Rogan, MT, Bodell, AJ and Craig, PS (2015) Post-encystment/established immunity in cystic echinococcosis: is it really that simple? Parasite Immunology 37, 1–9.Google Scholar
Romig, T, Ebi, D and Wassermann, M (2015) Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Veterinary Parasitology 213, 76–84.Google Scholar
Rothenberg, ME and Hogan, SP (2006) The eosinophil. Annual Review of Immunology 24, 147–174.Google Scholar
Sakamoto, T and Cabrera, PA (2003) Immunohistochemical observations on cellular response in unilocular hydatid lesions and lymph nodes of cattle. Acta Tropica 85, 271–279.Google Scholar
Siracusano, A, Delunardo, F, Teggi, A and Ortona, E (2012) Host–parasite relationship in cystic echinococcosis: an evolving story. Clinical and Developmental Immunology 2012, 639362. doi: 10.1155/2012/639362.Google Scholar
Tamarozzi, F, Mariconti, M, Neumayr, A and Brunetti, E (2016) The intermediate host immune response in cystic echinococcosis. Parasite Immunology 38, 170–181.Google Scholar
Thompson, RC (2017) Biology and systematics of Echinococcus. Advances in Parasitology 95, 65–109.Google Scholar
Ulrichs, T, Kosmiadi, GA, Trusov, V, Jörg, S, Pradl, L, Titukhina, M, Mishenko, V, Gushina, N and Kaufmann, SH (2004) Human tuberculous granulomas induce peripheral lymphoid follicle-like structures to orchestrate local host defence in the lung. The Journal of Pathology 204, 217–228.Google Scholar
Vatankhah, A, Halász, J, Piurkó, V, Barbai, T, Rásó, E and Tímár, J (2015) Characterization of the inflammatory cell infiltrate and expression of costimulatory molecules in chronic echinococcus granulosus infection of the human liver. BMC Infectious Diseases 15, 530. doi: 10.1186/s12879-015-1252-x.Google Scholar
Vismarra, A, Mangia, C, Passeri, B et al. (2015) Immuno-histochemical study of ovine cystic echinococcosis (Echinococcus granulosus) shows predominant T cell infiltration in established cysts. Veterinary Parasitology 209, 285–288.Google Scholar
Vivier, E, Raulet, DH, Moretta, A, Caligiuri, MA, Zitvogel, L, Lanier, LL, Yokoyama, WM and Ugolini, S (2011) Innate or adaptive immunity? The example of natural killer cells. Science 331, 44–49.Google Scholar
Wang, J, Müller, S, Lin, R, Siffert, M, Vuitton, DA, Wen, H and Gottstein, B (2017) Depletion of FoxP3+ Tregs improves control of larval Echinococcus multilocularis infection by promoting co-stimulation and Th1/17 immunity. Immunity, Inflammation and Disease 5, 435–447.Google Scholar
Wuestenberg, J, Gruener, B, Oeztuerk, S, Mason, RA, Haenle, MM, Graeter, T, Akinli, AS, Kern, P and Kratzer, W (2014) Diagnostics in cystic echinococcosis: serology versus ultrasonography. The Turkish Journal of Gastroenterology 25, 398–404.Google Scholar
Zhang, M, Wang, Z, Graner, MW et al. (2011) B cell infiltration is associated with the increased IL-17 and IL-22 expression in the lungs of patients with tuberculosis. Cellular Immunology 270, 217–223.Google Scholar
Zhang, W, Wen, H, Li, J, Lin, R and McManus, DP (2012) Immunology and immunodiagnosis of cystic echinococcosis: an update. Clinical and Developmental Immunology 2012, 101895. doi: 10.1155/2012/101895.Google Scholar
Supplementary material: File

Jafari et al. supplementary material

Tables S1-S3 and Figures S1-S6

Download Jafari et al. supplementary material(File)
File 4.7 MB