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Immunofluorescent studies in filariasis: antibody levels in rats infected with the Malayan forest filaria, Breinlia booliati*

Published online by Cambridge University Press:  05 June 2009

Mulkit Singh ,
G. B. Kane ,
Eu-Hian Yap ,
Beng-Chuan Ho ,
Joon-Wah Mak  and
Kim-Lian Kang
Mulkit Singh
Affiliation:
Department of Microbiology, Faculty of Medicine, University of Singapore, Singapore 3
G. B. Kane
Affiliation:
Department of Microbiology, Wellcome Research Laboratories, Beckenham, Kent, England
Eu-Hian Yap
Affiliation:
Department of Microbiology, Faculty of Medicine, University of Singapore, Singapore 3
Beng-Chuan Ho
Affiliation:
Department of Microbiology, Faculty of Medicine, University of Singapore, Singapore 3
Joon-Wah Mak
Affiliation:
Department of Microbiology, Division of Filariasis, Institute for Medical Research, Malaysia
Kim-Lian Kang
Affiliation:
Department of Microbiology, Faculty of Medicine, University of Singapore, Singapore 3
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Abstract

Antibody levels were investigated in rats infected with Breinlia booliati using the indirect immunofluorescent antibody technique. Sonicated antigens were prepared from microfilariae and adult worms. Adult worm antigen was rather unsatisfactory. The sonicated microfilarial antigen was useful in detecting antibodies in various groups of rat sera. Very high antibody levels were seen in amicrofilaraemic rats harbouring unisexual infections of varying worm loads. The singnificance of these observations are discussed.

Type
Research Papers
Information
Journal of Helminthology , Volume 54 , Issue 2 , June 1980 , pp. 155 - 160
Copyright
Copyright © Cambridge University Press 1980

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References

REFERENCES

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