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Immunodiagnosis of ocular toxocariasis using Western-blot for the detection of specific anti-Toxocara IgG and CAP™ for the measurement of specific anti-Toxocara IgE

Published online by Cambridge University Press:  12 April 2024

J.-F. Magnaval ,
L. Malard ,
B. Morassin  and
R. Fabre
J.-F. Magnaval*
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
L. Malard
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
B. Morassin
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
R. Fabre
Affiliation:
Service de Parasitologie, CHU Rangueil, 31403 Toulouse, 4, France
*
*Fax: 33 5 61 14 59 72 E-mail: [email protected].
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Abstract

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A prospective multicentric study was carried out to assess both the performance of Western-blot (WB) detecting specific anti-Toxocara IgG and that of CAP™ measuring specific IgE titre for the immunodiagnosis of ocular toxocariasis. For 14 outpatients presenting ophthalmic symptoms (choroiditis, chorioretinitis, papillar oedema, hyalitis, retinal detachment and/or uveitis), samples of serum and aqueous fluid (AF) were sent to the Department of Parasitology, University Hospitals, Toulouse, France. All patients but two tested positive with WB on the serum; 13 WB tests were performed on the AF, 12 of which were positive. The two patients who had a negative WB serum result tested positive for the AF. Specific IgE detection was considered as a complementary test of WB. Two patients showed a greater specific IgE titre in the AF than in the serum, and one had a positive result in the AF, but not in the serum. These six patients were considered as clear cases of ocular toxocariasis. Western-blot coupled with specific anti-Toxocara IgE detection appeared therefore to be an accurate procedure for the immunodiagnosis of ocular toxocariasis, provided the testing was simultaneously performed on the serum and AF.

Type
Research Article
Information
Journal of Helminthology , Volume 76 , Issue 4 , December 2002 , pp. 335 - 339
Copyright
Copyright © Cambridge University Press 2002

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