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Immunization of zebu calves against Fasciola gigantica, using irradiated metacercariae

Published online by Cambridge University Press:  05 June 2009

S. A. Younis ,
A. I. Yagi ,
E. M. Haroun ,
A. A. Gameel  and
M. G. Taylor
S. A. Younis
Affiliation:
Department of pathology, Faculty of Veterinary Science, University of Khartoum, P.O. Box 32, Khartoum North, Sudan
A. I. Yagi
Affiliation:
Department of pathology, Faculty of Veterinary Science, University of Khartoum, P.O. Box 32, Khartoum North, Sudan
E. M. Haroun
Affiliation:
Department of pathology, Faculty of Veterinary Science, University of Khartoum, P.O. Box 32, Khartoum North, Sudan
A. A. Gameel
Affiliation:
Department of pathology, Faculty of Veterinary Science, University of Khartoum, P.O. Box 32, Khartoum North, Sudan
M. G. Taylor
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, st. Albans, Herts AL4 0XQ, UK
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Abstract

The pathogenesis of unirradiated, 3 krad-irradiated and 20 krad-irradiated metacercarial infections was compared in zebu calves studied over a 10-week period. Calves exposed to 1000 unirradiated metacercariae (mc) became hypoalbuminaemic, and showed elevated serum concentrations of liver enzymes, whereas neither of the other groups was significantly affected. At slaughter, a mean of 332 flukes was recovered from the 0 krad group, while only 23% and 12% of this number were recovered from the 3 krad and 20 krad groups, respectively. All the worms recovered from the 20 krad group were stunted, and found in biliary ductules, but a mean of 13% of the flukes recovered from the 3 krad group were large, and dwelling in main bile-ducts. Liver lesions typical of acute fascioliasis were present in the 0 krad group, but lesions in the other groups, and particularly the 20 krad group, were far less severe. Judged on clinico-pathological criteria, a single vaccination of calves with 1000 3 krad-irradiated mc induced partial resistance to a challenge with 1000 normal mc eight weeks later, but the reduction in worm recovery was not statistically significant. There was less evidence of protection when two vaccinating doses of 3 krad mc were given within four weeks, with challenge at week 8, and a single vaccination was ineffective aganist a challenge four weeks later. However, when the irradiation dose was increased to 20 krad, a high level of resistance (69% worm reduction) was induced by a single vaccination, given eight weeks before challenge, and liver pathology was strikingly reduced in the vaccinated animals.

Keywords

Fasciola giganticaimmunizationcattleirradiated larvae
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 2 , June 1986 , pp. 123 - 134
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Armour, J., Dargie, J. D., Doyle, J. J., Murray, M., Robinson, P. & Rushton, B. (1974) Immunisation against fascioliasis. Proceedings of the Third International Congress of Parasitology, Munich, Vol. 1, 494.Google Scholar
Bitakaramire, P. K. (1973) Preliminary studies on the immunisation of cattle against fascioliasis using gamma-irradiated metacercariae of Fasciola gigantica. In: Isotopes and Radiation in Parasitology, III, I.A.E.A.: Vienna, pp. 23–32.Google Scholar
Boray, J. C. (1969) Experimental fascioliasis in Australia. Advances in Parasitology, 1, 95–210.Google Scholar
Boyd, J. W. (1962) The comparative activity of serum enzymes in sheep, cattle and rat in normal serum and tissue levels and changes during experimental liver necrosis. Research in Veterinary Science, 3, 256.Google Scholar
Ford, E.J.H. (1967) Activity of sorbitol dehydrogenase (SD) in the serum of sheep and cattle with liver damage. Journal of Comparative Pathology, 77, 405–411.Google Scholar
Ford, E.J.H. & Boyd, J. W. (1962) Cellular damage and changes in biliary excretion in a liver lesion of cattle. Journal of Pathology and Bacteriology, 83, 39–48.Google Scholar
Nansen, P. (1975) Resistance in cattle to Fasciola hepatica induced by X-ray attenuated larvae: results from a controlled field trial. Research in Veterinary Science, 19, 278–283.Google Scholar
Ogunrinade, A. F. (1980) The effect of gamma-irradiation on Fasciola gigantica infectivity to hamsters. International Journal of Applied Radiation and Isotopes, 31, 189.Google Scholar
Rodkey, F. L. (1965) Direct spectrophotometric determination of albumin in human serum. Clinical Chemistry, 11, 478–487.CrossRefGoogle ScholarPubMed
Sewell, M.M.H. (1966) The pathogenesis of fascioliasis. Veterinary Record, 78, 98–105.CrossRefGoogle ScholarPubMed
Sewell, M.M.H. (1967) Serum enzyme activity in acute ovine fascioliasis. Veterinary Record, 80, 577–578.Google Scholar
Sinclair, K. B. (1973) The resistance of sheep to Fasciola hepatica. Studies on the development and pathogenicity of challenge infections. British Veterinary Journal, 129, 236–250.CrossRefGoogle ScholarPubMed
Sinclair, K. B. (1975) Resistance of sheep to F. hepatica. Research in Veterinary Science, 19, 296–303.CrossRefGoogle Scholar
Taylor, M. G. (1980) Vaccination against trematodes. In: “Vaccination against Parasites”, British Society for Parasitology Symposium, 18, 115–140.Google Scholar
Weichselbaum, T. E. (1946) An accurate and rapid method for determination of proteins in small amounts of blood, serum and plasma. American Journal of Clinical Pathology. 16, 40–43.Google Scholar