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Immunization of mice against Trichinella spiralis and T. britovi using excretory and secretory antigens

Published online by Cambridge University Press:  05 June 2009

P.K. Goyal ,
F. Bolas-Fernandez  and
D. Wakelin
P.K. Goyal
Affiliation:
School of Studies in Zoology, Vikram University, Ujjain, 456 010, M.P., India:
F. Bolas-Fernandez
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Complutense University, Madrid, Spain:
D. Wakelin*
Affiliation:
Department of Life Science, University of Nottingham, Nottingham NG7 2RD, UK
*
* Author for correspondence.
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Abstract

Immune responses to immunization and infection with Trichinella spiralis and T. britovi were studied in NIH high-responder mice. Overall it was shown that T. britovi was the more immunogenic, immunization and challenge with this species giving greater host-protective immunity. This greater immunogenicity was reflected in higher proliferative responses when mesenteric node lymphocytes (MLNC) from immunized mice were restimulated with T. britovi antigens in vitro and in higher levels of T helper 2 (Th2) lymphocyte-dependent specific IgG1 antibody responses against this species. MLNC from mice immunized against T. britovi released more IL-5 when restimulated in vitro, again suggesting a greater T helper 2 subset response, but after infection the highest levels of IL-5 were recorded from MLNC taken from T. spiralis challenged mice. These data are discussed in relation to current understanding of immunological differences between species and isolates of the genus Trichinella.

Type
Symposium Papers
Information
Journal of Helminthology , Volume 71 , Issue 2 , June 1997 , pp. 109 - 112
Copyright
Copyright © Cambridge University Press 1997

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References

Bolas-Fernandez, F. & Wakelin, D. (1992) Immunization against geographical isolates of Trichinella spiralis in mice. International Journal for Parasitology 22, 773–781.CrossRefGoogle ScholarPubMed
Bolas-Fernandez, F., Albarran-Gomez, E., Navarrete, I. & Martinez-Fernandez, A.R. (1993) Dynamics of porcine humoral responses to experimental infections by Spanish Trichinella isolates: comparison of three larval antigens in ELISA. Journal of Veterinary Medicine 40, 229–238.CrossRefGoogle ScholarPubMed
Dea-Ayuela, M., Romaris, F., Arribas, B., Martinez-Fernandez, A.R., Ubeira, F. M. & Bolas-Fernandez, F. (1997) Identification of Trichinella species by the ELISA method using monoclonal antibodies. Proceedings of the 9th International Conference on Trichinellosis, Mexico (in press).Google Scholar
Else, K. J. & Grencis, R. K. (1991) Cellular immune responses to the murine nematode parasite Trichuris muris. 1. Differential cytokine production during acute or chronic infection. Immunology 72, 508–513.Google ScholarPubMed
Goyal, P. K. & Wakelin, D. (1993a) Influence of variation in host strain and parasite isolate on inflammatory and antibody responses to Trichinella spiralis in mice. Parasitology 106, 371–378.CrossRefGoogle ScholarPubMed
Goyal, P. K. & Wakelin, D. (1993b) Vaccination against Trichinella spiralis in mice using antigens from different isolates. Parasitology 107, 311–317.CrossRefGoogle ScholarPubMed
Goyal, P. K., Hermanek, J. & Wakelin, D. (1994) Lymphocyte proliferation and cytokine production in mice infected with different geographical isolates of Trichinella spiralis. Parasite Immunology 16, 105–110.CrossRefGoogle ScholarPubMed
Grencis, R. K., Riedlinger, J. & Wakelin, D. (1985) L3T4-positive T lymphoblasts are responsible for transfer of immunity to Trichinella spiralis in mice. Immunology 56, 213–218.Google ScholarPubMed
Grencis, R. K., Hultner, L. & Else, K. J. (1991) Host protective immunity to Trichinella spiralis in mice: activation of Th subsets and lymphokine secretion ion mice expressing different response phenotypes. Immunology 42, 329–332.Google Scholar
Pozio, E., La Rosa, G., Murrell, K. D. & Lichtenfels, J.R. (1992) Taxonomic revision of the genus Trichinella. Journal of Parasitology 78, 654–659.CrossRefGoogle ScholarPubMed
Silberstein, D. S. & Despommier, D. (1984) Antigens from Trichinella spiralis that induce a protective response in the mouse. Journal of Immunology 132, 898–904.CrossRefGoogle ScholarPubMed
Wakelin, D. & Goyal, P. K. (1996) Trichinella isolates: parasite variability and host responses. International Journal for Parasitology 26, 471–481.CrossRefGoogle ScholarPubMed
Wakelin, D. & Lloyd, M. (1976) Immunity to primary and challenge infection of Trichinella spiralis in mice: a reexamination of conventional parameters. Parasitology 72, 173–182.CrossRefGoogle ScholarPubMed
Wakelin, D., Goyal, P. K., Dehlawi, M. S. & Hermanek, J. (1994) Immune responses to Trichinella spiralis and Trichinella pseudospiralis in mice. Immunology 81, 475–479.Google ScholarPubMed