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Histopathological changes during experimental infections of calves with Cooperia punctata

Published online by Cambridge University Press:  12 April 2024

R.R. Rodrigues ,
S.M. Gennari ,
J.L. Guerra ,
M.B. Contieri ,
A.L. Abdalla  and
D.M.S.S. Vitti
R.R. Rodrigues
Affiliation:
Centro de Energia Nuclear na Agricultura, Avenida Centenário 303, CEP 13.400-970, Universidade de São Paulo, Piracicaba, Brazil, :
S.M. Gennari*
Affiliation:
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, SP, Brazil
J.L. Guerra
Affiliation:
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brazil
M.B. Contieri
Affiliation:
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brazil
A.L. Abdalla
Affiliation:
Centro de Energia Nuclear na Agricultura, Avenida Centenário 303, CEP 13.400-970, Universidade de São Paulo, Piracicaba, Brazil, :
D.M.S.S. Vitti
Affiliation:
Centro de Energia Nuclear na Agricultura, Avenida Centenário 303, CEP 13.400-970, Universidade de São Paulo, Piracicaba, Brazil, :
*
*Author for correspondence Fax: 11 3091 7928 Email: [email protected]
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Abstract

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Eleven male two-month-old Holstein calves were used to determine the pathological changes induced by a Cooperia punctata infection. After weaning, ten calves received a single oral dose of 45,000 C. punctata infective larvae. One calf remained as a non-infected control. Groups of two calves were killed on days 7, 14, 21, 28 and 35 post-infection (p.i.) for determination of worm burdens and histopathological evaluation. The small intestine was sub-divided into three sections of approximately equal length, and representative samples of mucosa were fixed in 10% formalin, cut, and stained with haematoxylin-eosin. Samples of intestinal contents and mucosal digests were taken and fixed in 10% formalin for an estimation of total worm burdens. An increase in the number of adult parasites and a decrease in the number of larvae were observed with time (P<0.001). A higher concentration of worms was found in the first segment of the small intestine during the five weeks of observation. Histology showed larvae in the intestinal mucosa on day 7 p.i., with a discrete increase in the cellular response. Adult worms and a marked cellular infiltrate with eosinophils and neutrophils were present on day 21 p.i., and these persisted until day 35 p.i. Microcysts resulting from worm destruction were observed from day 21 p.i.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 2 , June 2004 , pp. 167 - 171
Copyright
Copyright © Cambridge University Press 2004

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