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Histochemical and electrophoretic studies on phosphatases of some Indian trematodes

Published online by Cambridge University Press:  18 November 2009

Masoodul Haque  and
Ather H. Siddiqi
Masoodul Haque
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202 001, India
Ather H. Siddiqi
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202 001, India
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Abstract

The isoenzymes of acid and alkaline phosphatases and their histochemical localization were studied by polyacrylamide disc gel electrophoresis in four species of trematodes: Gigantocotyle explanatum from the liver and Gastrothylax crumenifer from the rumen of water buffalo (Bubalus bubalis) and Echinostoma malayanum and Fasciolopsis buski from the small intestine of the pig (Sus scrofa). Both acid and alkaline phosphatases were present in the tegument, gastrodermis, suckers, testes, ovary, eggs, vitellaria and uterus but alkaline phosphatase activity was demonstrated only in the parenchyma and excretory ducts. Polyacrylamide gel electrophoresis revealed two to four isoenzymes for both acid and alkaline phosphatase.

Type
Research Papers
Information
Journal of Helminthology , Volume 56 , Issue 2 , June 1982 , pp. 111 - 116
Copyright
Copyright © Cambridge University Press 1982

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