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Hepatic fibrosis and Fasciola hepatica infection in cattle

Published online by Cambridge University Press:  01 December 2007

Luis A. Marcos ,
Pedro Yi ,
Alfredo Machicado ,
Roy Andrade ,
Frine Samalvides ,
Juvenal Sánchez  and
Angélica Terashima
Luis A. Marcos*
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú Internal Medicine Department, University of Texas Health Science Center at Houston, Texas, USA
Pedro Yi
Affiliation:
Laboratorio de Patología, Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
Alfredo Machicado
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú
Roy Andrade
Affiliation:
Laboratorio de Patología, Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
Frine Samalvides
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú
Juvenal Sánchez
Affiliation:
Departamento de Patología, Instituto Nacional de Enfermedades, Neoplásicas, Lima, Perú
Angélica Terashima
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú
*
*E-mail: [email protected]
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Abstract

This study focuses on the development of fibrosis of the liver of cattle with Fasciola hepatica infection, correlating with the intensity of infection. Animals with an established diagnosis of chronic F. hepatica infection were identified in a slaughterhouse in Lima, Perú. The study included 24 fresh cattle livers from infected animals and two uninfected controls. Tissues were stored at 4°C for approximately 8 h after which they were brought to a necropsy room and examined. Between 9 and 12 biopsies were randomly obtained from each liver. Histological staining of formalin-fixed liver sections with haematoxylin and eosin (H & E) and Masson's trichrome were performed. Liver samples were examined using a pathology protocol that included 30 items. Histopathologically, 16 out of 30 liver specimens (67.6%) showed diffuse fibrotic lesions (cirrhosis) with a mean number of Fasciola of 116 ± 30 (range 4–435). Pathological data were matched to number of adult parasites and presence of cirrhosis after being reviewed by two independent pathologists. There was concordance between the two pathologists (K = 0.72). The group with cirrhosis showed an average of 116 ± 30 adult parasites whereas the group not showing cirrhosis contained 56 ± 28 flukes (P = 0.2). To measure how number of flukes and diagnosis of cirrhosis are related we used Kendall's tau-b coefficient; the correlation was +0.296 (P = 0.04). Receiver Operating Characteristic (ROC) curve results showed that the best point was 38 parasite adults, which had 93.8% sensitivity and 75% specificity. We conclude that as the number of F. hepatica adult forms increases, the likelihood of developing liver fibrosis will also increase in cattle.

Type
Research Papers
Information
Journal of Helminthology , Volume 81 , Issue 4 , December 2007 , pp. 381 - 386
Copyright
Copyright © Cambridge University Press 2007

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References

Alban, M., Jave, J. & Quispe, T. (2002) Fasciolais in Cajamarca. Revista Gastroenterologia del Perú 22 (1), 28–32.Google Scholar
Almendras-Jaramillo, M., Rivera-Medina, J., Seijas-Mogrovejo, J. & Almendras-Jaramillo, K. (1997) Hepatic fascioliasis in children: uncommon clinical manifestations. Arquivos de gastroenterologia 34 (4), 241–247.Google Scholar
Bakeman, R. & Gottman, J. (1986) Observación de la interacción: Introducción al análisis secuencial. Madrid, Morata.Google Scholar
Bonis, P., Friedman, S.L. & Kaplan, M.M. (2001) Is liver fibrosis reversible? New England Journal of Medicine 344, 452.Google Scholar
Braun, U., Jehle, W., Thio, T. & Pospischil, A. (2004) Case report: tenesmus in a cow with a liver abscess and nephritis. Deutsche Tierärztliche Wochenschrift 111 (1), 42–46.Google Scholar
Campbell, A.J., Sheers, M., Moore, R.J., Edwards, S.R. & Montague, E. (1981) Proline biosynthesis by Fasciola hepatica at different development stages in vivo and in vitro. Molecular and Biochemical Parasitology 3, 91–101.Google Scholar
Chen, M.G. & Mott, K.E. (1990) Progress in assessment of morbidity due to Fasciola hepatica infection. Tropical Disease Bulletin 87 (4) (Suppl.), R1–R38.Google Scholar
Dalton, J.P. (1999) Fasciolosis. pp. 185–199. Wallingford, UK, CABI Publishing.Google Scholar
De Aluja, A., Constantino, F. & Aldrete, J. (2002) Técnicas de Necropsia en animales domésticos. México, El Manual Moderno.Google Scholar
Friedman, S.L. (2002) Hepatic fibrosis: The consequences of liver disease. in Schiff, E.R., Sorrell, M.F. & Maddrey, W.C. (Eds) Schiff's diseases of the liver. 9th edn.London, Lippincott, Williams & Wilkins.Google Scholar
Hadden, J. & Pascarelli, E. (1967) Diagnosis and treatment of human fascioliasis. Journal of the American Medical Association 202 (2), 167–169.Google Scholar
Hamir, A.N. & Smith, B.B. (2002) Severe biliary hyperplasia with liver fluke infection in an adult Alpaca. Veterinary Pathology 39, 592–594.Google Scholar
Heredia, D., Bordas, J.M., Mondelo, F. & Rodes, J. (1984) Gallbladder fascioliasis in a patient with liver cirrhosis. Medicina Clinica (Barc.) 2 (17), 768–770.Google Scholar
Isseroff, H., Sawna, J.T. & Reino, D. (1977) Fascioliasis: role of proline in bile duct hyperplasia. Science 189, 1157–1159.Google Scholar
Jiménez, J., Loja, D., Ruiz, E., Maco, V., Marcos, L. & Aviles, R. (2001) Fasciolais hepática: un problema diagnóstico? Revista Gastroenterologia del Perú 21, 148–155.Google Scholar
Kim, J.B., Kim, D.J., Huh, S. & Cho, S.Y. (1995) A human case of invasive fascioliasis associated with liver abscess. Korean Journal of Parasitology 33 (4), 395–398.Google Scholar
Kim, K.A., Lim, H.K., Kim, S.H., Lee, W.J. & Lim, J.H. (1999) Necrotic granuloma of the liver by human fascioliasis: imaging findings. Abdominal Imaging 24 (5), 462–464.Google Scholar
Leguía, G., Alvarez, H., Náquira, F. & Beltrán, M. (1989) Distomatosis hepática en el Perú. pp. 96–107. Lima-Perú, Anales del Seminario Nacional de Zoonosis y Enfermedades de Transmisión Alimentaria.Google Scholar
Loja, D., Alvizuri, J., Vilca, M., Aviles, R. & Sanchez, M. (2003) Hepatic subcapsular hematoma caused by fascioliasis. Revista Gastroenterologia del Perú 23 (2), 142–148.Google Scholar
MacLean, L. (1999) Liver, lung, and intestinal fluke infections. pp. 1039–1057in Guerrant, R.L., Walker, D.H. & Weller, P.F. (Eds)Tropical infectious diseases: Principles, pathogens and practice. Philadelphia, Churchill Livingstone.Google Scholar
Marcos, L.A., Maco, V., Terashima, A., Samalvides, F. & Gotuzzo, E. (2002) Características clínicas de la infección crónica en niños. Revista Gastroenterologia del Perú 22 (3), 228–233.Google Scholar
Marcos, L.A., Maco, V., Terashima, A., Samalvides, F., Espinoza, J.R. & Gotuzzo, E. (2004) Hiperendemicidad de fasciolosis humana en el Valle del Mantaro: Factores de riesgo de la infección por Fasciola hepatica. Revista Gastroenterologia del Perú 24, 158–164.Google Scholar
Marcos, L.A., Maco, V., Castillo, M., Terashima, A., Zerpa, R. & Gotuzzo, E. (2005) Report of cases of human fascioliosis in the Specialized Children's Health Institute, Lima, Perú (1988–2003). Revista Gastroenterologia del Perú 25 (2), 198–205.Google Scholar
Mark, L.G. & Isseroff, H. (1983) Levels of type I and type III collagen in the bile duct of rats infected with Fasciola hepatica. Molecular and Biochemical Parasitology 8 (3), 253–262.Google Scholar
Oliveira, L., María de Souza, M. & Andrade, Z. (2004) Capillaria hepatica-induced hepatic fibrosis in rats: paradoxical effect of repeated infections. Revista da Sociedade Brasileira de Medicina Tropical 37 (2), 123–127.Google Scholar
Picoaga, J., Lopera, J. & Montes, J. (1980) Fasciolais en Arequipa. Boletin Peruano de Parasitología 2 (1–2), 1–11.Google Scholar
Regev, A., Berho, M. & Jeffers, L.J. (2002) Sampling error and intraobserver variation in liver biopsy in patients with chronic HCV infection. American Journal of Gastroenterology 97, 2614.Google Scholar
Sanchez-Sosa, S., Rojas-Ortega, S., Reed-San Roman, G. & Torres-Santana, M.A. (2000) Massive hepatobiliary fascioliasis. Revista Gastroenterologia. Mexicana 65 (4), 179–183.Google Scholar
Soloway, R.D., Baggenstoss, A.H., Schoenfield, L.J., et al. (1971) Observer error and sampling variability tested in evaluation of hepatitis and cirrhosis by liver biopsy. American Journal of Digestive Diseases 16, 1082.Google Scholar
Teichmann, D., Grobusch, M.P., Gobels, K., Muller, H.P., Koehler, W. & Suttorp, N. (2000) Acute fascioliasis with multiple liver abscesses. Scandinavian Journal of Infectious Diseases 32 (5), 558–560.Google Scholar
Valero, M., Santana, M., Hernandez, J.L. & Mas-Coma, S. (2003) Risk of gallstone disease in advanced chronic phase of fascioliasis: an experimental study in a rat model. Journal of Infectious Diseases 188, 787–793.Google Scholar
Vilchez, M. (1983) Anemia severa y fasciolasis crónica. Revista Gastroenterologia del Perú 2, 161–163.Google Scholar
Vitovec, J. (1974) Hepatocellular carcinoma in cattle and its relationship to biliary cirrhosis of fasciolar origin. Veterinary Pathology 11 (6), 548–557.Google Scholar
Wu, J. & Danielsson, A. (1995) Detection of hepatic fibrogenesis: a review of available techniques. Scandinavian Journal of Gastroenterology 30, 817.Google Scholar