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Helminth endoparasites of the smooth newt Lissotriton vulgaris: linking morphological identification and molecular data

Published online by Cambridge University Press:  05 March 2018

U. Sinsch ,
P. Heneberg ,
M. Těšínský ,
C. Balczun  and
P. Scheid
U. Sinsch*
Affiliation:
University of Koblenz-Landau, Department of Biology, Universitätsstrasse 1, D-56070 Koblenz, Germany
P. Heneberg
Affiliation:
Charles University, Third Faculty of Medicine, Ruská 87, CZ-100 00 Prague 10, Czech Republic
M. Těšínský
Affiliation:
Charles University, Third Faculty of Medicine, Ruská 87, CZ-100 00 Prague 10, Czech Republic
C. Balczun
Affiliation:
Laboratory of Medical Parasitology; Central Military Hospital Koblenz; Department XXI (Med. Microbiology); Andernacher Street 100; 56070 Koblenz, Germany
P. Scheid
Affiliation:
University of Koblenz-Landau, Department of Biology, Universitätsstrasse 1, D-56070 Koblenz, Germany Laboratory of Medical Parasitology; Central Military Hospital Koblenz; Department XXI (Med. Microbiology); Andernacher Street 100; 56070 Koblenz, Germany
*
Author for correspondence: U. Sinsch, E-mail: [email protected]
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Abstract

The helminth endoparasites of many European amphibian species are often known exclusively from morphological descriptions. A molecular library of DNA sequence data linked to morphological identifications is still in its infancy. In this paper, we aim to contribute to such a library on the smooth newt Lissotriton vulgaris, the intermediate and definitive host of 31 helminth parasites, according to evidence published so far. Newts (n = 69) were collected at two study sites in western Germany and examined for the presence of helminths. A total of five helminth species were detected in 56 (81%) of the newts, but only one or two species infected a single host. Four out of five helminth species were identified morphologically and based on DNA sequences as Parastrigea robusta (metacercariae), Oswaldocruzia filiformis, Megalobatrachonema terdentatum (adults and larvae) and Cosmocerca longicauda, and the corresponding sequences were provided subsequently. Oswaldocruzia molgeta was confirmed to be a junior synonym of O. filiformis. Molecular data on a fifth species (a cosmocercid nematode) that could not be identified at species level were added to GenBank. These findings increased the molecular library on morphologically identified smooth newt parasites significantly, from 12 to 15 entries.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 3 , May 2019 , pp. 332 - 341
Copyright
Copyright © Cambridge University Press 2018 

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