Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-22T19:31:54.274Z Has data issue: false hasContentIssue false
  • English
  • Français

Glucose 6-phosphate dehydrogenase: isoenzymatic pattern in Oesophagostomum venulosum, Trichuris ovis and T. suis

Published online by Cambridge University Press:  05 June 2009

B. Rodriguez ,
C. Cutillas ,
P. German  and
D. Guevara
B. Rodriguez
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Sor Gregoria de Santa Teresa S/N, 41012-Sevilla, Spain
C. Cutillas
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Sor Gregoria de Santa Teresa S/N, 41012-Sevilla, Spain
P. German
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Sor Gregoria de Santa Teresa S/N, 41012-Sevilla, Spain
D. Guevara
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Sor Gregoria de Santa Teresa S/N, 41012-Sevilla, Spain
Get access Rights & Permissions [Opens in a new window]

Abstract

In the present communication we have studied the isoenzymatic pattern activity of the glucose 6-phosphate dehydrogenase (G6PD) in Oesophagostomum venulosum, Trichuris ovis and T. suis, parasites of Capra hircus (goat), Ovis aries (sheep) and Sus scrofa domestica (pig) respectively, by polyacrylamide gel electrophoresis. Different phenotypes have been observed in the G6PD isoenzymatic pattern activity in males and females of Oesophagostomum venulosum. Furthermore, G6PD activity has been assayed in Trichuris ovis collected from Ovis aries and Capra hircus. No differences have been observed in the isoenzymatic patterns attending to the different hosts. All the individuals exhibited one single band or two bands; this suggests a monomeric condition for G6PD in T. ovis. In T. suis the enzyme G6PD appeared as a single electrophoretic band in about 85·7% of the individuals.

Keywords

Nematodapolyacrylamide gel electrophoresisisoenzymesOesophagostomum venulosumTrichuris ovisTrichuris suis
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 4 , December 1991 , pp. 289 - 295
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Agatsuma, T. (1981) Electrophoretic demonstration of polymorphism of glucose phosphate isomerase in natural populations of Paragonimus miyazakii. Journal of Parasitology, 67, 452–454.CrossRefGoogle ScholarPubMed
Agatsuma, T. & Suzuki, N. (1981) Electrophoretic studies on enzymes in Paragonimus spp. I. Comparison of isozyme patterns between P. ohirai and P. miyazakii. Japanese Journal of Parasitology, 30, 37–43.Google Scholar
Avise, J. C. (1976) Systematic value of electrophoretic data. Systematic Zoology, 23, 465–481.CrossRefGoogle Scholar
Fletcher, M. & Loverde, P. T. (1981) Schistosoma mansoni: Electrophoretic characterization of strains selected for different levels of infectivity to snails. Experimental Parasitology, 52, 362–370.CrossRefGoogle ScholarPubMed
Hames, B. D. & Rickwood, D. (1981) Gel Electrophoresis of Proteins. IRL Press Limited, Oxford, England.Google Scholar
Kamada, T. & Hori, S. H. (1970) A phylogenic study of animal glucose 6-phosphate dehydrogenases. Japanese Journal of Genetics, 45, 319–339.Google Scholar
Lymbery, A. J. & Thompson, R. C. A. (1988) Electrophoretic analysis of genetic variation in Echinococcus granulosus from domestic hosts in Australia. International Journal for Parasitology, 18, 803–811.CrossRefGoogle ScholarPubMed
Novak, M., Taylor, W. R. & Pip, E. (1988) Interspecific variation of isoenzyme patterns in four Hymenolepis species (Cestoda). Canadian Journal of Zoology, 67, 2052–2055.CrossRefGoogle Scholar
Ohnishi, K. (1985) Constant rate of evolution in the antigenicity of glucose 6-phosphate dehydrogenase revealed by the enzyme inhibition method. Comparative Biochemistry and Physiology, 80B, 217–222.Google ScholarPubMed
Ortega, J. E. & Valero, A. (1989) Diferenciación genética entre dos poblaciones del complejo Bothriocephalus scorpii (Pseudophyllidea: Bothriocephalidae). Revista Ibérica de Parasitología, 49, 139–145.Google Scholar
Paggi, L., Nascetti, G., Orecchia, P., Mattiucci, S. & Bullini, L. (1985) Biochemical taxonomy of ascaridoid nematodes. Parassitologia, 27, 105–112.Google ScholarPubMed
Pasteur, N., Pasteur, G., Bonhomme, F., Catalan, J. & Britton-Davidian, J. (1988) Practical Isozyme Genetics. Ellis Horwood Series in Gene technology.Google Scholar
Rawn, J. D. (1989) Bioquimica. McGraw Hill. Interamericana de España. 1a Ed. Madrid, España.Google Scholar
Shaw, C. R. & Prassad, R. (1970) Starch gel electrophoresis of enzymes-A compilation of recipes. Biochemical Genetics, 4, 297–320.CrossRefGoogle ScholarPubMed
Strickberger, M. W. (1978) Genetica. 2nd Ed., Ed. Omega, S.A., Barcelona.Google Scholar
Yoshida, A. A. (1975) Evolution of glucose 6-phosphate dehydrogenase and hexose 6-phosphate dehydrogenase. In: Isozymes, IV, Genetics and Evolution, pp. 853–866, Academic Press, San Francisco.Google Scholar
Zee, D. S. & Zinkham, W. H. (1968) Malate dehydrogenase in Ascaris suum: characterization, ontogenic, and genetic control. Archives of Biochemistry and Biophysics, 126, 574–584.CrossRefGoogle Scholar