Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-19T05:12:29.088Z Has data issue: false hasContentIssue false
  • English
  • Français

Filarial DNA and its interaction with polyamines and antifilarial drugs

Published online by Cambridge University Press:  05 June 2009

U. Pandya ,
J.K. Saxena  and
O.P. Shukla
U. Pandya
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow 226 001, India
J.K. Saxena
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow 226 001, India
O.P. Shukla
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow 226 001, India
Get access Rights & Permissions [Opens in a new window]

Abstract

The interaction of DNA from filarial parasite Setaria cervi with polyamines was monitored by melting temperature (Tm) profile, condensation and B to Z DNA transition and compared with DNA of Escherichia coli, Micrococcus luteus and calf thymus having different GC content. Polyamines, viz. spermine and spermidine, stabilized the secondary structure of all DNAs as indicated by increase in Tm value. UV absorption studies indicated B to Z DNA transition in the presence of polyamines. The amount of polyamines required for B to Z transition was dependent upon base composition of DNA and charge of the polyamine. Filarial DNA (AT rich) required six times higher concentration of spermine as compared to GC rich DNA for B to Z DNA transition. Spermidine was not effective in causing transition of S. cervi DNA even at Spd:DNA-P ratio of 20. The antifilarial compound suramin significantly decreased melting temperature of filarial DNA as compared to GC rich DNAs of other parasites. Suramin adversely affected condensation and B to Z DNA transition of various DNAs but prior addition of polyamines protected the DNAs from the destabilizing effect of suramin.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 4 , December 1997 , pp. 325 - 332
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Balasundram, D. & Tyagi, A.K. (1991) Polyamine DNA nexus structural ramification and biological implication. Molecular and Cellular Biology 10, 129–140.Google Scholar
Bancroft, D., Williams, L.D., Rich, A. & Egli, M. (1994) Low temperature crystal structure of the purine-spermine form of Z DNA reveals binding of a spermine molecule in the minor groove. Biochemistry 33, 1073–1086.CrossRefGoogle ScholarPubMed
Basu, H.S. & Marton, L.J. (1987) The interaction of spermine and pentamines with DNA. Biochemical journal 244, 243–246.CrossRefGoogle ScholarPubMed
Behe, M. & Felsenfeld, G. (1981) Effects of methylation on synthetic polynucleotides: The B-Z transition in poly (dG-m5dC) poly (dG-m5dC). Proceedings of the National Academy of Sciences, USA 78, 1619–1623.CrossRefGoogle ScholarPubMed
Drew, H.R. & Dickerson, R.E. (1981) Structure of a B-DNA dodecamer. III. Geometries of hydration. Journal of Molecular Biology 151, 535–556.Google Scholar
Duschak, U.G. & Goldenberg, S.H. (1987) Effects of DNA gyrase inhibitors in a polyamine auxotrophic strain of E. coli. Biochimica et Biophysica Acta 910, 21–26.CrossRefGoogle Scholar
Feuerstein, B.G., Pattabiraman, M. & Marton, L.J. (1986) Spermine-DNA interactions: a theoretical study. Proceedings of the National Academy of Sciences USA 83, 5948–5952.CrossRefGoogle ScholarPubMed
Hasan, R., Alam, M.K. & Ali, R. (1995) Polyamines induced Z conformation of native calf thymus DNA. FEES Letters 368, 27–30.Google Scholar
Mandel, M. & Marmur, J. (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12B, 195–206.CrossRefGoogle Scholar
Marquet, R. & Houssier, C. (1988) Different binding modes of spermine to A–T and G–C base pairs modulate the bending and stiffening of the DNA double helix. Journal of Biomolecular and Structural Dynamics 6, 235–246.CrossRefGoogle Scholar
Marmur, J. (1963) A procedure for isolation of DNA from microorganisms. Methods in Enzymology 6, 726–738.Google Scholar
Marton, L.J. & Pegg, A.E. (1995) Polyamines as targets for therapeutic intervention. Annual Review of Pharmacology and Toxicology 33, 55–91.CrossRefGoogle Scholar
Morris, D.R. & Handa, J.J. (1980) pp. 1–15in Gaugas, J.M. (Ed.) Polyamines in biomedical research. London, John Wiley & Sons.Google Scholar
Padmanabhan, S., Brushabes, V.M., Anderson, C.F. & Record, M.T. Jr. (1991) Relative affinities of divalent polyamines and their N-methylated analogues for helical DNA determined by 23 Na NMR. Biochemistry 30, 7550–7559.Google Scholar
Pandya, U., Roy, U. & Shukla, O.P. (1991) Isolation and characterization of DNA from some helminth parasites. Medical Science Research 19, 619–620.Google Scholar
Pandya, U. & Shukla, O.P. (1994) Suramin destabilizes secondary structure of filarial DNA. Medical Science Research 22, 833–834.Google Scholar
Pandya, U., Saxena, J.K. & Shukla, O.P. (1994) Effect of ivermectin on thermal denaturation of filarial DNA. Indian Journal of Parasitology 18, 121–126.Google Scholar
Pegg, A.E. (1986) Recent advances in the biochemistry of polyamine in eukaryotes. Biochemical Journal 234, 249–262.Google Scholar
Pohl, P.M. & Jovin, T.M. (1972) Salt induced conformational changes of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC). Journal of Molecular Biology 67, 375–396.Google Scholar
Rodger, A., Blagbrough, I.S., Adlam, G. & Carpenter, M.L. (1994) DNA binding of a spermine derivative: spectroscopic study of anthracine-9-carbonyl-N-spermine with poly[d(G-C). d(G-C)] and poly [d(A-T). d(A-T)]. Biopolymers 34, 1583–1594.Google Scholar
Rodger, A., Taylor, S., Adlam, G., Blagbrough, I.S. & Haworth, I.S. (1995) Multiple DNA binding modes of anthracine-9-C-N-spermine. Bioinorganic and Medicinal Chemistry 3, 861–872.Google Scholar
Rothstein, N., Stoller, T.J. & Rajan, T.V. (1988) DNA base composition of filarial nematodes. Parasitology 97, 75–79.Google Scholar
Schmid, N. & Behr, J.P. (1991) Location of spermine and other polyamines on DNA as revealed by photo affinity cleavage with polyamino benzene diazonium salt. Biochemistry 30, 4357–4361.Google Scholar
Sharma, V., Tekwani, B.L., Saxena, J.K., Gupta, S., Katiyar, J.C., Chatterjee, R.K., Ghatak, S. & Shukla, O.P. (1991) Polyamine metabolism in some helminth parasites. Experimental Parasitology 72, 15–23.CrossRefGoogle ScholarPubMed
Singh, R.P., Saxena, J.K., Ghatak, S., Shukla, O.P, Wittich, R.M. & Walter, R.D. (1989) Polyamine metabolism in Setaria cervi, the bovine filarial worm. Parasitology Research 75, 311–315.Google Scholar
Tabor, C.W. & Tabor, H. (1984) Polyamines. Annual Review of Biochemistry 53, 749–790.Google Scholar
Thomas, T.J. & Bloomfield, V.A. (1985) Toroidal condensation of Z DNA and identification of an intermediate in the B–Z transition of poly (dG-m5dC). Poly (dG-m5dC). Biochemistry 24, 713–719.CrossRefGoogle Scholar
Thomas, T.J. & Messner, R.P. (1988) Structural specificity and polyamines in left-handed Z-DNA formation. Journal of Molecular Biology 201, 463–467.CrossRefGoogle ScholarPubMed
Walter, R.D. (1988) Polyamine metabolism of filarial and allied parasites. Parasitology Today 4, 18–20.Google Scholar
Wemmer, D.E., Srivenugopal, K.S., Reid, B.R. & Morris, D.R. (1985) Nuclear magnetic resonance studies of polyamines binding to defined DNA sequence. Journal of Molecular Biology 185, 457–459.Google Scholar
Williams, L.D., Frederick, C.A., Ughetto, G. & Rich, A. (1990) Ternary interactions of spermine with DNA:4'-epiadramycin and other DNA:anthracycline complexes. Nucleic Acids Research 18, 5533–5542.Google Scholar
Yuki, M., Grukhin, V., Lee, C-S. & Haworth, I.S. (1996) Spermine binding to GC rich DNA experimental and theoretical studies. Archives of Biochemistry and Biophysics 325, 39–46.Google Scholar