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Fertilization state of Ascaris suum determined by electrorotation

Published online by Cambridge University Press:  12 April 2024

C. Dalton ,
A.D. Goater  and
H.V. Smith
C. Dalton*
Affiliation:
Bio Systems Research and Applications Group, Department of Electrical and Computer Engineering, University of Calgary, Calgary, T2N 1N4, Canada
A.D. Goater
Affiliation:
Institute of Bioelectronic and Molecular Microsystems, University of Wales, Bangor, Gwynedd, LL57 1UT, UK
H.V. Smith
Affiliation:
Scottish Parasite Diagnostic Laboratory, Stobhill Hospital, Springburn, Glasgow, G21 3UW, UK.
*
* Fax: 403 282 6855 E-mail: [email protected].
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Abstract

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Electrorotation is a non-invasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. The first detailed electrorotation study of the egg (ovum) of a parasitic nematode, namely Ascaris suum is described to show that electrorotation can rapidly differentiate between fertilized and non-fertilized eggs. Support for this conclusion is by optical microscopy of egg morphology, and also from modelling of the electrorotational response. Modelling was used to determine differences in the dielectric properties of the unfertilized and fertilized eggs, and also to investigate specific differences in the spectra of fertilized eggs only, potentially reflecting embryogenesis. The potential of electrorotation as an investigative tool is shown, as undamaged eggs can be subjected to further non-destructive and destructive techniques, which could provide further insight into parasite biology and epidemiology.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 1 , March 2006 , pp. 25 - 31
Copyright
Copyright © Cambridge University Press 2006

Footnotes

†

Where the work was performed

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