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Faecal egg counts provide a reliable measure of Trichostrongylus tenuis intensities in free-living red grouse Lagopus lagopus scoticus

Published online by Cambridge University Press:  12 April 2024

L.J. Seivwright ,
S.M. Redpath ,
F. Mougeot ,
L. Watt  and
P.J. Hudson
L.J. Seivwright*
Affiliation:
CEH Banchory, Hill of Brathens, Glassel, Banchory, Aberdeenshire, AB31 4BW, UK, : Department of Biological and Molecular Sciences, University of Stirling, Stirling, FK9 4LA, UK, :
S.M. Redpath
Affiliation:
CEH Banchory, Hill of Brathens, Glassel, Banchory, Aberdeenshire, AB31 4BW, UK, :
F. Mougeot
Affiliation:
CEH Banchory, Hill of Brathens, Glassel, Banchory, Aberdeenshire, AB31 4BW, UK, :
L. Watt
Affiliation:
BioSciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK, :
P.J. Hudson
Affiliation:
Biology Department, Mueller Laboratory, Penn State University, University Park, PA 16802, USA
*
*Fax: 01330 823303 Email: [email protected]
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Abstract

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The reliability of different egg counting methods for estimating the intensity of Trichostrongylus tenuis infections in red grouse, Lagopus lagopus scoticus, was investigated in the autumn, when grouse may harbour high parasite intensities. Possible limitations to the use of these methods were also examined. Faecal egg counts were found to accurately estimate T. tenuis worm intensities, at least up to an observed maximum of c. 8000 worms. Two egg counting methods (smear and McMaster) gave consistent results, although the exact relationship with worm intensity differed according to the method used. Faecal egg counts significantly decreased with increasing length of sample storage time, but egg counts were reliable for estimating worm intensity for three weeks. The concentration of eggs in the caecum was also found to reliably estimate worm intensity. However, egg counts from frozen gut samples cannot be used to estimate worm intensities. These results conclude that, despite some limitations, faecal and caecum egg counts provide useful and reliable ways of measuring T. tenuis intensities in red grouse.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 1 , March 2004 , pp. 69 - 76
Copyright
Copyright © Cambridge University Press 2004

References

Albon, S.D., Stien, A., Irvine, R.J., Langvatn, R., Ropstad, E. & Halvorsen, O. (2002) The role of parasites in the dynamics of a reindeer population. Proceedings of the Royal Society of London Series B–Biological Sciences 269, 1625–1632.CrossRefGoogle ScholarPubMed
Anderson, R.M. (1978) The regulation of host population growth by parasite species. Parasitology 76, 119–157.CrossRefGoogle Scholar
Anderson, R.M. & Schad, G.A. (1985) Hookworm burdens and faecal egg counts: an analysis of the biological basis of variation. Transactions of the Royal Society of Tropical Medicine and Hygiene 79, 812–825.CrossRefGoogle ScholarPubMed
Barger, I.A. (1987) Population regulation in trichostrongylids of ruminants. pp. 531–539 in Howell, M.J. (Ed.) Parasitology - Quo Vadit? Proceedings of the Sixth International Congress of Parasitology. Canberra, Australian Academy of Science.Google Scholar
Callinan, A.P.L. & Arundel, J.H. (1982) Population dynamics of the parasite stages of Ostertagia spp. in sheep. International Journal for Parasitology 12, 531–535.CrossRefGoogle ScholarPubMed
Chiejina, S.N., Sewell, M.M.H. (1974) Worm burdens, acquired resistance and live weight gains in lambs during prolonged daily infections with Trichostrongylus colubriformis (Giles 1982) Loos. Parasitology 69, 315–327.CrossRefGoogle Scholar
Coadwell, W.J., Ward, P.F.V. (1982) The use of faecal egg counts for estimating worm burdens in sheep infected with Haemonchus contortus . Parasitology 85, 251–256.CrossRefGoogle ScholarPubMed
Coop, R.L., Graham, R.B., Jackson, F., Wright, S.E. & Angus, K.W. (1985) Effect of experimental Ostertagia circumcincta infection on the performance of grazing lambs. Research in Veterinary Science 38, 282–287.CrossRefGoogle ScholarPubMed
Elkins, D.B., Sithithaworn, P., Haswell-Elkins, M., Kaewkes, S., Awacharagan, P. & Wongratanacheewin, S. (1991) Opisthorchis viverrini : relationships between egg counts, worms recovered and antibody levels within a community in northeast Thailand. Parasitology 102, 283–288.CrossRefGoogle ScholarPubMed
Gasaway, W.C., White, R.G. & Holleman, D.F. (1976) Digestion of dry matter and absorption of water in the intestine and caecum of rock ptarmigan. Condor 78, 77–84.CrossRefGoogle Scholar
Gibson, T.E. & Parfitt, J.W. (1973) The development of resistance to Trichostrongylus colubriformis by lambs under conditions of continuous infection. Research in Veterinary Science 15, 220–223.CrossRefGoogle ScholarPubMed
Gordon, H., McL., & Whitlock, H.V. (1939) A new technique for counting nematode eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research (Australia) 12, 50–52.Google Scholar
Gulland, F.M.D. & Fox, M. (1992) Epidemiology of nematode infections of Soay sheep (Ovis aries, L.) on St Kilda. Parasitology 105, 481–492.CrossRefGoogle Scholar
Guyatt, H.L., Bundy, D.A.P. (1993) Estimation of intestinal nematode prevalence: influence of parasite mating patterns. Parasitology 107, 99–106.CrossRefGoogle ScholarPubMed
Hudson, P.J. (1986a) The effect of a parasitic nematode on the breeding production of red grouse. Journal of Animal Ecology 55, 85–92.CrossRefGoogle Scholar
Hudson, P.J. (1986b) Red grouse: the biology and management of a wild gamebird. Fordingbridge, UK, The Game Conservancy Trust.Google Scholar
Hudson, P.J. & Dobson, A.P. (1997) Transmission dynamics and host-parasite interactions of Trichostrongylus tenuis in red grouse (Lagopus lagopus scoticus). Journal of Parasitology 83, 194–202.CrossRefGoogle ScholarPubMed
Hudson, P.J. & Newborn, D. (1995) A handbook of grouse and moorland management. Fordingbridge, UK, The Game Conservancy Trust.Google Scholar
Hudson, P.J., Dobson, A.P. & Newborn, D. (1985) Cyclical and non-cyclic populations of red grouse: a role for parasitism? pp. 77–89 in Rollinson, D. & Anderson, R.M. (Eds) Ecology and genetics of host parasite infections. London, Academic Press.Google Scholar
Hudson, P.J., Dobson, A.P. & Newborn, D. (1998) Prevention of population cycles by parasite removal. Science 282, 2256–2258.CrossRefGoogle ScholarPubMed
Hudson, P.J., Newborn, D. & Dobson, A.P. (1992) Regulation and stability of a free-living host-parasite system: Trichostrongylus tenuis in red grouse. I. Monitoring and parasite reduction experiments. Journal of Animal Ecology 61, 477–486.CrossRefGoogle Scholar
Irvine, R.J., Stien, A., Dallas, J.F., Halvorsen, O., Langvatn, R. & Albon, S.D. (2001) Contrasting regulation of fecundity in two abomasal nematodes of Svalbard reindeer (Rangifer tarandus platyrhynchus). Parasitology 122, 673–681.CrossRefGoogle ScholarPubMed
Le Jambre, L.F., Ratcliffe, L.H., Uhazy, L.S. & Whitlock, J.H. (1971) Faecal egg output of lambs in relation to Haemonchus contortus burden. International Journal for Parasitology 1, 157–160.CrossRefGoogle Scholar
Leopold, A.S. (1953) Intestinal morphology of gallinaceous birds in relation to food habits. Journal of Wildlife Management 17, 197–203.CrossRefGoogle Scholar
Littell, R.C., Milliken, G.A., Stroup, W.W. & Wolfinger, R.D. (1996) SAS system for mixed models. Cary, North Carolina, SAS Institute Inc.Google Scholar
MAFF (1986) Manual of veterinary parasitological laboratory techniques. Part 1 Helminthology. Reference Book No. 418 Ministry of Agriculture, Fisheries and Food.Google Scholar
May, R.M. & Anderson, R.M. (1978) Regulation and stability of host-parasite population interactions Part II. Destabilising processes. Journal of Animal Ecology 47, 249–268.CrossRefGoogle Scholar
Michael, E., Bundy, D.A.P. (1989) Density dependence in establishment, growth and worm fecundity in intestinal helminthiasis: the population biology of Trichuris muris (Nematoda) infection in CBA/Ca mice. Parasitology 98, 451–458.CrossRefGoogle ScholarPubMed
Michel, J.F. (1963) The phenomena of host resistance and the course of infection of Ostertagia ostertagia in calves. Parasitology 53, 63–84.CrossRefGoogle Scholar
Michel, J.F. (1969) Some observations on the worm burdens of calves infected daily with Ostertagia ostertagia . Parasitology 59, 575–595.CrossRefGoogle Scholar
Moss, R. & Parkinson, J.A. (1972) The digestion of heather (Calluna vulgaris) by red grouse (Lagopus lagopus scoticus). British Journal of Nutrition 27, 285–298.CrossRefGoogle ScholarPubMed
Moss, R., Trenholm, I.B., Watson, A. & Parr, R. (1990) Parasitism, predation and survival of hen red grouse Lagopus lagopus scoticus in spring. Journal of Animal Ecology 59, 631–642.CrossRefGoogle Scholar
Moss, R., Watson, A., Trenholm, I.B. & Parr, R. (1993) Caecal threadworms Trichostrongylus tenuis in red grouse Lagopus lagopus scoticus : effects of weather and host density upon estimated worm burdens. Parasitology 107, 119–209.CrossRefGoogle ScholarPubMed
Roberts, J.L. & Swan, R.A. (1981) Quantitative studies of ovine haemonchosis 1. Relationship between faecal egg counts and total worm counts. Veterinary Parasitology 8, 165–171.CrossRefGoogle Scholar
SAS Institute (2001) SAS/STAT users’ guide, version 8.01. Cary, North Carolina, SAS Institute Inc.Google Scholar
Shaw, J.L. (1988a) Arrested development of Trichostrongylus tenuis as third stage larvae in red grouse. Research in Veterinary Science 45, 256–258.CrossRefGoogle ScholarPubMed
Shaw, J.L. (1988b) Epidemiology of the caecal threadworm Trichostrongylus tenuis in red grouse (Lagopus lagopus scoticus). Unpublished PhD thesis, University of Aberdeen.Google Scholar
Shaw, J.L. & Moss, R. (1989a) The role of parasite fecundity and longevity in the success of Trichostrongylus tenuis in low density red grouse populations. Parasitology 99, 253–258.CrossRefGoogle ScholarPubMed
Shaw, J.L. & Moss, R. (1989b) Factors affecting the establishment threadworm Trichostrongylus tenuis in red grouse (Lagopus lagopus scoticus). Parasitology 99, 259–264.CrossRefGoogle ScholarPubMed
Shaw, J.L., Moss, R. & Pike, A.W. (1989) Development and survival of the free-living stages of Trichostrongylus tenuis, a caecal parasite of red grouse Lagopus lagopus scoticus . Parasitology 99, 105–113.CrossRefGoogle ScholarPubMed
Sinniah, B. (1982) Daily egg production of Ascaris lumbricoides : the distribution of eggs in the faeces and the variability of egg counts. Parasitology 84, 167–175.CrossRefGoogle ScholarPubMed
Sithithaworn, P., Tesana, S., Pipitgool, V., Kaewkes, S., Pairojkul, C., Sripa, B., Paupairoj, A. & Thaiklar, K. (1991) Relationship between faecal egg count and worm burden of Opisthorchis viverrini in human autopsy cases. Parasitology 102, 277–281.CrossRefGoogle ScholarPubMed
Smith, G., Grenfell, B.T. & Anderson, R.M. (1987) The regulation of Ostertagia ostertagi populations in calves: density dependent control of fecundity. Parasitology 95, 373–388.CrossRefGoogle ScholarPubMed
Tompkins, D.M. & Hudson, P.J. (1999) Regulation of nematode fecundity in the ring-necked pheasant (Phasianus colchicus): not just density dependence. Parasitology 118, 417–423.CrossRefGoogle Scholar
Watson, H., Lee, D.L. & Hudson, P.J. (1988) Primary and secondary infections of the domestic chicken with Trichostrongylus tenuis (Nematoda), a parasite of red grouse, with observations on the effect of the caecal mucosa. Parasitology 97, 89–99.CrossRefGoogle ScholarPubMed
Wilson, G.R. (1979) Effects of the caecal threadworm Trichostrongylus tenuis on red grouse. Unpublished PhD thesis, University of Aberdeen.Google Scholar
Wilson, G.R. (1983) The effects of caecal threadworm (Trichostrongylus tenuis) in red grouse (Lagopus lagopus scoticus). Oecologia 58, 265–268.CrossRefGoogle Scholar