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Evaluation of anthelmintic activity of Iris hookeriana against gastrointestinal nematodes of sheep

Published online by Cambridge University Press:  01 June 2008

K.A. Tariq ,
M.Z. Chishti ,
F. Ahmad ,
A.S. Shawl  and
M.A. Tantray
K.A. Tariq*
Affiliation:
Post Graduate Department of Zoology, The University of Kashmir, Srinagar-190 006, Jammu and Kashmir, India
M.Z. Chishti
Affiliation:
Post Graduate Department of Zoology, The University of Kashmir, Srinagar-190 006, Jammu and Kashmir, India
F. Ahmad
Affiliation:
Post Graduate Department of Zoology, The University of Kashmir, Srinagar-190 006, Jammu and Kashmir, India
A.S. Shawl
Affiliation:
Indian Institute of Integrative Medicine, Council of Scientific and Industrial Research, Sanat Nagar, Srinagar-190 005, Jammu and Kashmir, India
M.A. Tantray
Affiliation:
Indian Institute of Integrative Medicine, Council of Scientific and Industrial Research, Sanat Nagar, Srinagar-190 005, Jammu and Kashmir, India
*
*E-mail: [email protected]
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Abstract

The objective of this study was to evaluate the anthelmintic efficacy of Iris hookeriana Linn. rhizome against gastrointestinal nematodes of sheep. A worm motility inhibition assay was used for in vitro study and a faecal egg count reduction assay was used for an in vivo study. The in vitro study revealed anthelmintic effects of crude aqueous extracts and crude ethanolic extracts on live Trichuris ovis worms (P ≤ 0.05) as evident from their paralysis and/or death at 8 h after exposure. The aqueous extracts of I. hookeriana resulted in a mean worm motility inhibition of 54.0%, while ethanolic extracts resulted in a mean worm motility inhibition of 84.6%. The mean mortality index of aqueous extracts was 0.55, while for ethanolic extracts it was 0.85. The lethal concentration 50 for aqueous extracts was 0.45 mg ml− 1 and for ethanolic extracts it was 0.15 mg ml− 1. The in vivo anthelmintic activity of aqueous and ethanolic extracts of I. hookeriana in sheep naturally infected with mixed species of gastrointestinal nematodes demonstrated a maximum (45.62%) egg count reduction in sheep treated with ethanolic extracts at 2 g kg− 1 body weight on day 10 after treatment, closely followed by ethanolic extracts at 1 g kg− 1 body weight on day 10 after treatment (43.54% egg count reduction). The aqueous extracts resulted in a maximum of 31.53% reduction in faecal egg counts on day 10 after treatment with 1 g kg− 1 body weight. Thus ethanolic extracts exhibited greater anthelmintic activity under both in vitro and in vivo conditions; this could be due to the presence of alcohol-soluble active ingredients in I. hookeriana. From the present study it can be suggested that I. hookeriana rhizome exhibited significant anthelmintic activity against gastrointestinal nematodes of sheep and has the potential to contribute to the control of gastrointestinal nematode parasites of small ruminants.

Type
Research Papers
Information
Journal of Helminthology , Volume 82 , Issue 2 , June 2008 , pp. 135 - 141
Copyright
Copyright © Cambridge University Press 2008

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