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Eosinophilia, IL-5 level and recovery of larvae in IL-5 transgenic mice infected with Toxocara canis

Published online by Cambridge University Press:  05 June 2009

K. Sugane ,
Y. Kusama ,
M. Takamoto ,
A. Tominaga  and
K. Takatsu
K. Sugane
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City 390, Japan
Y. Kusama
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City 390, Japan
M. Takamoto
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City 390, Japan
A. Tominaga
Affiliation:
Department of Medical Biology, Kochi Medical School, Kochi, Japan
K. Takatsu
Affiliation:
Department of Immunology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
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Abstract

Eosinophil counts, interleukin 5 (IL-5) concentrations in peripheral blood and larval recovery after infection with Toxocara canis were compared between IL-5 transgenic (Tg) and nontransgenic counterparts, C3H/HeN mice. In Tg mice characterized by a constant high level of peripheral eosinophils, eosinophils in peripheral blood fell to the lowest level on day 4 of a T. canis infection and then returned to the preinfection level on day 14. Serum IL-5 level fell to the lowest level on day 4 of infection, then recovered rapidly and peaked on day 14 postinfection. In contrast, the eosinophil and IL-5 levels in the peripheral blood peaked on days 11 and 7 of infection, respectively in C3H/HeN mice. The degree of eosinophil infiltration into the lung 4 days after infection was far more pronounced in Tg than C3H/HeN mice. The highest number of larvae recovered from the lungs of infected Tg and C3H/HeN mice occurred on day 4 postinfection. Strains of Tg and C3H/HeN mice vaccinated with excretory and secretory (ES) antigens of T. canis larvae were infected with T. canis and the recovery of larvae on day 21 analysed. There were no significant differences in the mean number of larvae recovered from nonvaccinated Tg and C3H/HeN mice or vaccinated Tg and C3H/HeN mice. However, significant differences were demonstrated in the mean total number of larvae recovered from vaccinated and nonvaccinated Tg or C3H/HeN mice. These results suggest that immunocompetent cells other than eosinophils may play a significant role in the expulsion and killing of T. canis larvae in infected mice.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 2 , June 1996 , pp. 153 - 158
Copyright
Copyright © Cambridge University Press 1996

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