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Enzyme-linked immunosorbent assay with worm vomit and cercarial secretions of Schistosoma mansoni to detect infections in an endemic focus of Burkina Faso

Published online by Cambridge University Press:  12 April 2024

M. Bahgat ,
H. Sorgho ,
J.B. Ouédraogo ,
J.N. Poda ,
L. Sawadogo  and
A. Ruppel
M. Bahgat
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
H. Sorgho
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso
J.B. Ouédraogo
Affiliation:
Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso
J.N. Poda
Affiliation:
Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso
L. Sawadogo
Affiliation:
UFR-SVT, Université de Ouagadougou, Burkina Faso.
A. Ruppel*
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
*
* Author for correspondence Fax: +49 6221 565948, E-mail: [email protected].
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Abstract

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Cercariae and adult Schistosoma mansoni were used to prepare, respectively, cercarial secretions (CS) and worm vomit (WoV). These were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to test the IgG-reactivity of sera obtained in an S. mansoni-endemic area of Burkina Faso. Among the egg-excreting individuals (n=240), 94.6% reacted positively with WoV, but only 62.9% with CS, thus suggesting a high diagnostic sensitivity of WoV, but not of CS. Among those individuals without detectable eggs in two Kato-Katz thick smears from different stool specimens (n=215), the respective percentages of positive IgG reactivity were 78.1% and 63.3%. These positive reactions in the absence of detectable eggs are interpreted in terms of limited sensitivity of parasitological stool examinations. Optical density values in ELISA with CS, but not with WoV, correlated negatively with age, which may reflect decreasing exposure to cercariae in older individuals.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 1 , March 2006 , pp. 19 - 23
Copyright
Copyright © Cambridge University Press 2006

Footnotes

†

Department of Medicinal Chemistry and Central Laboratory, Division of Pharmaceutical Industries and Drug Research, National Research Center, Dokki, Cairo, Egypt.

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