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ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosisin pigs naturally infected with Taenia solium

Published online by Cambridge University Press:  11 April 2024

A. Ito ,
A. Plancarte ,
M. Nakao ,
K. Nakaya ,
T. Ikejima ,
Z.X. Piao ,
T. Kanazawa  and
S.S. Margono
A. Ito*
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa, 780, Japan
A. Plancarte
Affiliation:
Departamento de Parasitologia y Microbiologia Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico, 04510, Mexico D.F.
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa, 780, Japan
K. Nakaya
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, 780, Japan
T. Ikejima
Affiliation:
Research Center of Emergency Treating Drugs, Affiliated Hospital of Changchun College of Traditional Chinese Medicine, Changchun, 130021, China
Z.X. Piao
Affiliation:
Research Center of Emergency Treating Drugs, Affiliated Hospital of Changchun College of Traditional Chinese Medicine, Changchun, 130021, China
T. Kanazawa
Affiliation:
Department of Parasitology and Tropical Medicine, University of Occupational and Environmental Health, Japan Kitakyushu, 807, Japan
S.S. Margono
Affiliation:
Department of Parasitology Faculty of Medicine, University of Indonesia, Jakarta, 10430, Indonesia
*
*Fax: +81 166 68 2429 E-mail: [email protected]
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Abstract

The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected with Taenia solium. All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones.

Type
Research Article
Information
Journal of Helminthology , Volume 73 , Issue 4 , April 1999 , pp. 363 - 365
Copyright
Copyright © Cambridge University Press 1999

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